Abstract
Synthetic biology designed cell-free biosensors are a promising new tool for the detection of clinically relevant biomarkers in infectious diseases. Here, we report that a modular DNA-encoded biosensor in cell-free protein expression systems can be used to measure a bacterial biomarker of Pseudomonas aeruginosa infection from human sputum samples. By optimizing the cell-free system and sample extraction, we demonstrate that the quorum sensing molecule 3-oxo-C12-HSL in sputum samples from cystic fibrosis lungs can be quantitatively measured at nanomolar levels using our cell-free biosensor system, and is comparable to LC-MS measurements of the same samples. This study further illustrates the potential of modular cell-free biosensors as rapid, low-cost detection assays that can inform clinical practice.
| Original language | English |
|---|---|
| Pages (from-to) | 2293-2301 |
| Number of pages | 9 |
| Journal | ACS Synthetic Biology |
| Volume | 6 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 15 2017 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2017 American Chemical Society.
ASJC Scopus Subject Areas
- Biomedical Engineering
- Biochemistry, Genetics and Molecular Biology (miscellaneous)
Keywords
- biosensor
- cell-free synthetic biology
- cystic fibrosis
- Pseudomonas aeruginosa
- quorum sensing
