A Dual-Mode NADH Biosensor Based on Gold Nanostars Decorated CoFe2 Metal-Organic Frameworks to Reveal Dynamics of Cell Metabolism

Xiaoping Zhao; Ruoxin Niu; Shu Fan; Xunan Jing; Rui Gao; Hongbo Yang; Heng Wang; Daquan Wang; Zhiwei Yang; Yunchuan Xie; Junjun She; Peng Chen; Lingjie Meng

doi:10.1021/acssensors.2c01175

A Dual-Mode NADH Biosensor Based on Gold Nanostars Decorated CoFe₂ Metal-Organic Frameworks to Reveal Dynamics of Cell Metabolism

Xiaoping Zhao, Ruoxin Niu, Shu Fan, Xunan Jing, Rui Gao, Hongbo Yang, Heng Wang, Daquan Wang, Zhiwei Yang, Yunchuan Xie, Junjun She, Peng Chen^*, Lingjie Meng^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Nicotinamide adenine dinucleotide (NADH) is central to metabolism and implicated in various diseases. Herein, nanohybrids of gold nanostars and metal-organic frameworks are devised and demonstrated as a dual-mode NADH sensor, for which colorimetric detection is enabled by its peroxidase-like nanozyme property and Raman detection is realized by its surface-enhanced Raman scattering property with the detection limit as low as 28 pM. More importantly, this probe enables real-time SERS monitoring in living cells, providing a unique tool to investigate dynamic cellular processes involving NADH. Our experiments reveal that metabolism dynamics is accelerated by glucose and is much higher in cancerous cells. The SERS results can also be verified by the colorimetric detection. This sensor provides a new potential to detect biomarkers and their dynamics in situ.

Original language	English
Pages (from-to)	2671-2679
Number of pages	9
Journal	ACS Sensors
Volume	7
Issue number	9
DOIs	https://doi.org/10.1021/acssensors.2c01175
Publication status	Published - Sept 23 2022
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2022 American Chemical Society. All rights reserved.

ASJC Scopus Subject Areas

Bioengineering
Instrumentation
Process Chemistry and Technology
Fluid Flow and Transfer Processes

Keywords

cell metabolism
gold nanostars
metal-organic frameworks (MOFs)
peroxidase-like
reduced nicotinamide adenine dinucleotide (NADH)
surface-enhanced Raman scattering (SERS)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1021/acssensors.2c01175

Cite this

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title = "A Dual-Mode NADH Biosensor Based on Gold Nanostars Decorated CoFe2 Metal-Organic Frameworks to Reveal Dynamics of Cell Metabolism",

abstract = "Nicotinamide adenine dinucleotide (NADH) is central to metabolism and implicated in various diseases. Herein, nanohybrids of gold nanostars and metal-organic frameworks are devised and demonstrated as a dual-mode NADH sensor, for which colorimetric detection is enabled by its peroxidase-like nanozyme property and Raman detection is realized by its surface-enhanced Raman scattering property with the detection limit as low as 28 pM. More importantly, this probe enables real-time SERS monitoring in living cells, providing a unique tool to investigate dynamic cellular processes involving NADH. Our experiments reveal that metabolism dynamics is accelerated by glucose and is much higher in cancerous cells. The SERS results can also be verified by the colorimetric detection. This sensor provides a new potential to detect biomarkers and their dynamics in situ.",

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author = "Xiaoping Zhao and Ruoxin Niu and Shu Fan and Xunan Jing and Rui Gao and Hongbo Yang and Heng Wang and Daquan Wang and Zhiwei Yang and Yunchuan Xie and Junjun She and Peng Chen and Lingjie Meng",

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T1 - A Dual-Mode NADH Biosensor Based on Gold Nanostars Decorated CoFe2 Metal-Organic Frameworks to Reveal Dynamics of Cell Metabolism

AU - Zhao, Xiaoping

AU - Niu, Ruoxin

AU - Fan, Shu

AU - Jing, Xunan

AU - Gao, Rui

AU - Yang, Hongbo

AU - Wang, Heng

AU - Wang, Daquan

AU - Yang, Zhiwei

AU - Xie, Yunchuan

AU - She, Junjun

AU - Chen, Peng

AU - Meng, Lingjie

PY - 2022/9/23

Y1 - 2022/9/23

N2 - Nicotinamide adenine dinucleotide (NADH) is central to metabolism and implicated in various diseases. Herein, nanohybrids of gold nanostars and metal-organic frameworks are devised and demonstrated as a dual-mode NADH sensor, for which colorimetric detection is enabled by its peroxidase-like nanozyme property and Raman detection is realized by its surface-enhanced Raman scattering property with the detection limit as low as 28 pM. More importantly, this probe enables real-time SERS monitoring in living cells, providing a unique tool to investigate dynamic cellular processes involving NADH. Our experiments reveal that metabolism dynamics is accelerated by glucose and is much higher in cancerous cells. The SERS results can also be verified by the colorimetric detection. This sensor provides a new potential to detect biomarkers and their dynamics in situ.

AB - Nicotinamide adenine dinucleotide (NADH) is central to metabolism and implicated in various diseases. Herein, nanohybrids of gold nanostars and metal-organic frameworks are devised and demonstrated as a dual-mode NADH sensor, for which colorimetric detection is enabled by its peroxidase-like nanozyme property and Raman detection is realized by its surface-enhanced Raman scattering property with the detection limit as low as 28 pM. More importantly, this probe enables real-time SERS monitoring in living cells, providing a unique tool to investigate dynamic cellular processes involving NADH. Our experiments reveal that metabolism dynamics is accelerated by glucose and is much higher in cancerous cells. The SERS results can also be verified by the colorimetric detection. This sensor provides a new potential to detect biomarkers and their dynamics in situ.

KW - cell metabolism

KW - gold nanostars

KW - metal-organic frameworks (MOFs)

KW - peroxidase-like

KW - reduced nicotinamide adenine dinucleotide (NADH)

KW - surface-enhanced Raman scattering (SERS)

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