A high-performance liquid chromatography and nuclear magnetic resonance spectroscopy-based analysis of commercially available praziquantel tablets

Jia Li; Yulan Wang; Alan Fenwick; T. Andrew Clayton; Yu Y.K. Lau; Cristina Legido-Quigley; John C. Lindon; Jürg Utzinger; Elaine Holmes

doi:10.1016/j.jpba.2007.06.017

A high-performance liquid chromatography and nuclear magnetic resonance spectroscopy-based analysis of commercially available praziquantel tablets

Jia Li, Yulan Wang, Alan Fenwick, T. Andrew Clayton, Yu Y.K. Lau, Cristina Legido-Quigley, John C. Lindon, Jürg Utzinger, Elaine Holmes^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

The amount of active ingredient in 20 commercially sourced batches of praziquantel (PZQ) tablets was determined using a high-performance liquid chromatography-ultraviolet (HPLC-UV) assay in conjunction with an anthentic, lot of PZQ powder. The general composition of each batch of tablets was also examined by means of ¹H nuclear magnetic resonance (NMR) spectroscopy and the NMR data were subjected to pattern recognition analysis by means of principal component analysis. The HPLC-UV results showed that each batch of PZQ tablets contained approximately the required amount of PZQ (600 mg per tablet). The NMR analysis showed a high degree of compositional variation between manufacturers, which caused by variation in excipients, along with some batch-to-batch variation in the tablets from a single manufacturer. Additionally, the PZQ tablets from one manufacturer were found to have an extra component (methyl-4-hydroxybenzoate) that was not detected in the other preparations.

Original language	English
Pages (from-to)	263-267
Number of pages	5
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	45
Issue number	2
DOIs	https://doi.org/10.1016/j.jpba.2007.06.017
Publication status	Published - Oct 18 2007
Externally published	Yes

ASJC Scopus Subject Areas

Analytical Chemistry
Pharmaceutical Science
Drug Discovery
Spectroscopy
Clinical Biochemistry

Keywords

High-performance liquid chromatography
Nuclear magnetic resonance spectroscopy
Praziquantel
Principal component analysis
Quality control
Schistosomiasis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jpba.2007.06.017

Cite this

Li, J., Wang, Y., Fenwick, A., Clayton, T. A., Lau, Y. Y. K., Legido-Quigley, C., Lindon, J. C., Utzinger, J., & Holmes, E. (2007). A high-performance liquid chromatography and nuclear magnetic resonance spectroscopy-based analysis of commercially available praziquantel tablets. Journal of Pharmaceutical and Biomedical Analysis, 45(2), 263-267. https://doi.org/10.1016/j.jpba.2007.06.017

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title = "A high-performance liquid chromatography and nuclear magnetic resonance spectroscopy-based analysis of commercially available praziquantel tablets",

abstract = "The amount of active ingredient in 20 commercially sourced batches of praziquantel (PZQ) tablets was determined using a high-performance liquid chromatography-ultraviolet (HPLC-UV) assay in conjunction with an anthentic, lot of PZQ powder. The general composition of each batch of tablets was also examined by means of 1H nuclear magnetic resonance (NMR) spectroscopy and the NMR data were subjected to pattern recognition analysis by means of principal component analysis. The HPLC-UV results showed that each batch of PZQ tablets contained approximately the required amount of PZQ (600 mg per tablet). The NMR analysis showed a high degree of compositional variation between manufacturers, which caused by variation in excipients, along with some batch-to-batch variation in the tablets from a single manufacturer. Additionally, the PZQ tablets from one manufacturer were found to have an extra component (methyl-4-hydroxybenzoate) that was not detected in the other preparations.",

keywords = "High-performance liquid chromatography, Nuclear magnetic resonance spectroscopy, Praziquantel, Principal component analysis, Quality control, Schistosomiasis",

author = "Jia Li and Yulan Wang and Alan Fenwick and Clayton, {T. Andrew} and Lau, {Yu Y.K.} and Cristina Legido-Quigley and Lindon, {John C.} and J{\"u}rg Utzinger and Elaine Holmes",

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doi = "10.1016/j.jpba.2007.06.017",

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Li, J, Wang, Y, Fenwick, A, Clayton, TA, Lau, YYK, Legido-Quigley, C, Lindon, JC, Utzinger, J & Holmes, E 2007, 'A high-performance liquid chromatography and nuclear magnetic resonance spectroscopy-based analysis of commercially available praziquantel tablets', Journal of Pharmaceutical and Biomedical Analysis, vol. 45, no. 2, pp. 263-267. https://doi.org/10.1016/j.jpba.2007.06.017

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AU - Li, Jia

AU - Wang, Yulan

AU - Fenwick, Alan

AU - Clayton, T. Andrew

AU - Lau, Yu Y.K.

AU - Legido-Quigley, Cristina

AU - Lindon, John C.

AU - Utzinger, Jürg

AU - Holmes, Elaine

PY - 2007/10/18

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AB - The amount of active ingredient in 20 commercially sourced batches of praziquantel (PZQ) tablets was determined using a high-performance liquid chromatography-ultraviolet (HPLC-UV) assay in conjunction with an anthentic, lot of PZQ powder. The general composition of each batch of tablets was also examined by means of 1H nuclear magnetic resonance (NMR) spectroscopy and the NMR data were subjected to pattern recognition analysis by means of principal component analysis. The HPLC-UV results showed that each batch of PZQ tablets contained approximately the required amount of PZQ (600 mg per tablet). The NMR analysis showed a high degree of compositional variation between manufacturers, which caused by variation in excipients, along with some batch-to-batch variation in the tablets from a single manufacturer. Additionally, the PZQ tablets from one manufacturer were found to have an extra component (methyl-4-hydroxybenzoate) that was not detected in the other preparations.

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KW - Nuclear magnetic resonance spectroscopy

KW - Praziquantel

KW - Principal component analysis

KW - Quality control

KW - Schistosomiasis

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A high-performance liquid chromatography and nuclear magnetic resonance spectroscopy-based analysis of commercially available praziquantel tablets

Abstract

ASJC Scopus Subject Areas

Keywords

UN SDGs

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