Abstract
Astrocytes exhibit dynamic Ca2+ mobilization, such as Ca 2+ wave and Ca2+ oscillation, via an inositol 1,4,5-triphosphate-induced Ca2+ release (IICR)-dependent mechanism. The physiological functions of astrocytic Ca2+ mobilization, however, are poorly understood. To investigate this issue, we created a plasmid encoding an enhanced green fluorescent protein-tagged inositol 1,4,5-triphosphate absorbent protein and expressed it in cultured astrocytes. Expression of this protein inhibited both IICR and the Ca2+ wave in cultured astrocytes. By combining this method to the single cell electroporation technique, we were able to inhibit IICR specifically in astrocytes in an astrocyte-neuron co-culture system. Our approach provides a useful tool for direct examination of the physiological role of astrocytic Ca2+ signaling on neuronal function.
Original language | English |
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Pages (from-to) | 196-201 |
Number of pages | 6 |
Journal | Brain Research |
Volume | 1055 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - Sept 7 2005 |
Externally published | Yes |
ASJC Scopus Subject Areas
- General Neuroscience
- Molecular Biology
- Clinical Neurology
- Developmental Biology
Keywords
- Astrocyte
- Calcium wave
- IP3
- IP3 scavenger
- Single cell electroporation