A proteomics analysis of cellular proteins associated with HBV genotype-specific HBX: Potential in identification of early diagnostic markers for HCC

Background: Hepatocellular carcinoma (HCC) is one of the most common cancers in the world and causes approximately one million deaths every year. HCC is highly prevalent in Asia and closely associated with chronic hepatitis B virus (HBV) infection, with HBX protein playing a key role in the hepatocarcinogenic process. In addition, HBV genotypes B and C are clinically associated with different outcome of infection. Reliable biomarkers for early diagnosis and prognosis of HCC are needed for effective treatment. Objectives: We propose to establish a proteomics approach to identify cellular proteins associated with HBX of a particular genotype. Study design: Genotype-specific HBXs are used to transfect non-HCC Chang cells. Transfected cell extracts are analyzed by proteomics approach to identify cellular proteins associated with specific HBX. Results: A number of cellular proteins have been found to be specifically associated with HBX of genotype B or C. Conclusions: Our results suggest that proteomics approach could be used to identify cellular proteins associated with HBV infection of specific genotype. Similar approach could be deployed in the identification of molecular markers for early diagnosis of HCC.