A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies

Patthara Kongsuphol; Huan Jia; Hoi Lok Cheng; Yue Gu; Bhuvaneshwari D./O Shunmuganathan; Ming Wei Chen; Sing Mei Lim; Say Yong Ng; Paul Ananth Tambyah; Haziq Nasir; Xiaohong Gao; Dousabel Tay; Seunghyeon Kim; Rashi Gupta; Xinlei Qian; Mary M. Kozma; Kiren Purushotorman; Megan E. McBee; Paul A. MacAry; Hadley D. Sikes; Peter R. Preiser

doi:10.1038/s43856-021-00045-9

A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies

Patthara Kongsuphol, Huan Jia, Hoi Lok Cheng, Yue Gu, Bhuvaneshwari D./O Shunmuganathan, Ming Wei Chen, Sing Mei Lim, Say Yong Ng, Paul Ananth Tambyah, Haziq Nasir, Xiaohong Gao, Dousabel Tay, Seunghyeon Kim, Rashi Gupta, Xinlei Qian, Mary M. Kozma, Kiren Purushotorman, Megan E. McBee, Paul A. MacAry^*, Hadley D. Sikes^*Peter R. Preiser^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Background: Neutralizing antibodies (NAbs) prevent pathogens from infecting host cells. Detection of SARS-CoV-2 NAbs is critical to evaluate herd immunity and monitor vaccine efficacy against SARS-CoV-2, the virus that causes COVID-19. All currently available NAb tests are lab-based and time-intensive. Method: We develop a 10 min cellulose pull-down test to detect NAbs against SARS-CoV-2 from human plasma. The test evaluates the ability of antibodies to disrupt ACE2 receptor—RBD complex formation. The simple, portable, and rapid testing process relies on two key technologies: (i) the vertical-flow paper-based assay format and (ii) the rapid interaction of cellulose binding domain to cellulose paper. Results: Here we show the construction of a cellulose-based vertical-flow test. The developed test gives above 80% sensitivity and specificity and up to 93% accuracy as compared to two current lab-based methods using COVID-19 convalescent plasma. Conclusions: A rapid 10 min cellulose based test has been developed for detection of NAb against SARS-CoV-2. The test demonstrates comparable performance to the lab-based tests and can be used at Point-of-Care. Importantly, the approach used for this test can be easily extended to test RBD variants or to evaluate NAbs against other pathogens.

Original language	English
Article number	46
Journal	Communications Medicine
Volume	1
Issue number	1
DOIs	https://doi.org/10.1038/s43856-021-00045-9
Publication status	Published - Dec 2021
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2021, The Author(s).

ASJC Scopus Subject Areas

Public Health, Environmental and Occupational Health
Internal Medicine
Epidemiology
Medicine (miscellaneous)
Assessment and Diagnosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/s43856-021-00045-9

Cite this

Kongsuphol, P., Jia, H., Cheng, H. L., Gu, Y., Shunmuganathan, B. D. O., Chen, M. W., Lim, S. M., Ng, S. Y., Tambyah, P. A., Nasir, H., Gao, X., Tay, D., Kim, S., Gupta, R., Qian, X., Kozma, M. M., Purushotorman, K., McBee, M. E., MacAry, P. A., ... Preiser, P. R. (2021). A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies. Communications Medicine, 1(1), Article 46. https://doi.org/10.1038/s43856-021-00045-9

@article{150099b6168c48cb880ba9552e048752,

title = "A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies",

abstract = "Background: Neutralizing antibodies (NAbs) prevent pathogens from infecting host cells. Detection of SARS-CoV-2 NAbs is critical to evaluate herd immunity and monitor vaccine efficacy against SARS-CoV-2, the virus that causes COVID-19. All currently available NAb tests are lab-based and time-intensive. Method: We develop a 10 min cellulose pull-down test to detect NAbs against SARS-CoV-2 from human plasma. The test evaluates the ability of antibodies to disrupt ACE2 receptor—RBD complex formation. The simple, portable, and rapid testing process relies on two key technologies: (i) the vertical-flow paper-based assay format and (ii) the rapid interaction of cellulose binding domain to cellulose paper. Results: Here we show the construction of a cellulose-based vertical-flow test. The developed test gives above 80% sensitivity and specificity and up to 93% accuracy as compared to two current lab-based methods using COVID-19 convalescent plasma. Conclusions: A rapid 10 min cellulose based test has been developed for detection of NAb against SARS-CoV-2. The test demonstrates comparable performance to the lab-based tests and can be used at Point-of-Care. Importantly, the approach used for this test can be easily extended to test RBD variants or to evaluate NAbs against other pathogens.",

author = "Patthara Kongsuphol and Huan Jia and Cheng, {Hoi Lok} and Yue Gu and Shunmuganathan, {Bhuvaneshwari D./O} and Chen, {Ming Wei} and Lim, {Sing Mei} and Ng, {Say Yong} and Tambyah, {Paul Ananth} and Haziq Nasir and Xiaohong Gao and Dousabel Tay and Seunghyeon Kim and Rashi Gupta and Xinlei Qian and Kozma, {Mary M.} and Kiren Purushotorman and McBee, {Megan E.} and MacAry, {Paul A.} and Sikes, {Hadley D.} and Preiser, {Peter R.}",

note = "Publisher Copyright: {\textcopyright} 2021, The Author(s).",

year = "2021",

month = dec,

doi = "10.1038/s43856-021-00045-9",

language = "English",

volume = "1",

journal = "Communications Medicine",

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Kongsuphol, P, Jia, H, Cheng, HL, Gu, Y, Shunmuganathan, BDO, Chen, MW, Lim, SM, Ng, SY, Tambyah, PA, Nasir, H, Gao, X, Tay, D, Kim, S, Gupta, R, Qian, X, Kozma, MM, Purushotorman, K, McBee, ME, MacAry, PA, Sikes, HD & Preiser, PR 2021, 'A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies', Communications Medicine, vol. 1, no. 1, 46. https://doi.org/10.1038/s43856-021-00045-9

TY - JOUR

T1 - A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies

AU - Kongsuphol, Patthara

AU - Jia, Huan

AU - Cheng, Hoi Lok

AU - Gu, Yue

AU - Shunmuganathan, Bhuvaneshwari D./O

AU - Chen, Ming Wei

AU - Lim, Sing Mei

AU - Ng, Say Yong

AU - Tambyah, Paul Ananth

AU - Nasir, Haziq

AU - Gao, Xiaohong

AU - Tay, Dousabel

AU - Kim, Seunghyeon

AU - Gupta, Rashi

AU - Qian, Xinlei

AU - Kozma, Mary M.

AU - Purushotorman, Kiren

AU - McBee, Megan E.

AU - MacAry, Paul A.

AU - Sikes, Hadley D.

AU - Preiser, Peter R.

PY - 2021/12

Y1 - 2021/12

N2 - Background: Neutralizing antibodies (NAbs) prevent pathogens from infecting host cells. Detection of SARS-CoV-2 NAbs is critical to evaluate herd immunity and monitor vaccine efficacy against SARS-CoV-2, the virus that causes COVID-19. All currently available NAb tests are lab-based and time-intensive. Method: We develop a 10 min cellulose pull-down test to detect NAbs against SARS-CoV-2 from human plasma. The test evaluates the ability of antibodies to disrupt ACE2 receptor—RBD complex formation. The simple, portable, and rapid testing process relies on two key technologies: (i) the vertical-flow paper-based assay format and (ii) the rapid interaction of cellulose binding domain to cellulose paper. Results: Here we show the construction of a cellulose-based vertical-flow test. The developed test gives above 80% sensitivity and specificity and up to 93% accuracy as compared to two current lab-based methods using COVID-19 convalescent plasma. Conclusions: A rapid 10 min cellulose based test has been developed for detection of NAb against SARS-CoV-2. The test demonstrates comparable performance to the lab-based tests and can be used at Point-of-Care. Importantly, the approach used for this test can be easily extended to test RBD variants or to evaluate NAbs against other pathogens.

AB - Background: Neutralizing antibodies (NAbs) prevent pathogens from infecting host cells. Detection of SARS-CoV-2 NAbs is critical to evaluate herd immunity and monitor vaccine efficacy against SARS-CoV-2, the virus that causes COVID-19. All currently available NAb tests are lab-based and time-intensive. Method: We develop a 10 min cellulose pull-down test to detect NAbs against SARS-CoV-2 from human plasma. The test evaluates the ability of antibodies to disrupt ACE2 receptor—RBD complex formation. The simple, portable, and rapid testing process relies on two key technologies: (i) the vertical-flow paper-based assay format and (ii) the rapid interaction of cellulose binding domain to cellulose paper. Results: Here we show the construction of a cellulose-based vertical-flow test. The developed test gives above 80% sensitivity and specificity and up to 93% accuracy as compared to two current lab-based methods using COVID-19 convalescent plasma. Conclusions: A rapid 10 min cellulose based test has been developed for detection of NAb against SARS-CoV-2. The test demonstrates comparable performance to the lab-based tests and can be used at Point-of-Care. Importantly, the approach used for this test can be easily extended to test RBD variants or to evaluate NAbs against other pathogens.

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A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies

Abstract

Bibliographical note

ASJC Scopus Subject Areas

UN SDGs

Access to Document

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Simple New 10-Minute Test Accurately Detects COVID-19 Immunity

Ten-minute test for detection of COVID immunity

Cite this

A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies

Abstract

Bibliographical note

ASJC Scopus Subject Areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Press/Media

Simple New 10-Minute Test Accurately Detects COVID-19 Immunity

Ten-minute test for detection of COVID immunity

Cite this