TY - JOUR
T1 - A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes
AU - Goldberg, Susanne M.D.
AU - Johnson, Justin
AU - Busam, Dana
AU - Feldblyum, Tamara
AU - Ferriera, Steve
AU - Friedman, Robert
AU - Halpern, Aaron
AU - Khouri, Hoda
AU - Kravitz, Saul A.
AU - Lauro, Federico M.
AU - Li, Kelvin
AU - Rogers, Yu Hui
AU - Strausberg, Robert
AU - Sutton, Granger
AU - Tallon, Luke
AU - Thomas, Torsten
AU - Venter, Eli
AU - Frazier, Marvin
AU - Venter, J. Craig
PY - 2006/7/25
Y1 - 2006/7/25
N2 - Since its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for producing cost-effective and high-quality genome sequence data. Until now, the Sanger sequencing technology that has served as a platform for WGS has not been truly challenged by emerging technologies. The recent introduction of the pyrosequencing-based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS. In this study, we evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.
AB - Since its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for producing cost-effective and high-quality genome sequence data. Until now, the Sanger sequencing technology that has served as a platform for WGS has not been truly challenged by emerging technologies. The recent introduction of the pyrosequencing-based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS. In this study, we evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.
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U2 - 10.1073/pnas.0604351103
DO - 10.1073/pnas.0604351103
M3 - Article
C2 - 16840556
AN - SCOPUS:33746605947
SN - 0027-8424
VL - 103
SP - 11240
EP - 11245
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 30
ER -
