A study of osteocalcin production in vitro on various hydroxyapatite based biomaterials

J. L. Xu; K. A. Khor; W. N. Chen

doi:10.4028/0-87849-422-7.897

A study of osteocalcin production in vitro on various hydroxyapatite based biomaterials

J. L. Xu^*, K. A. Khor, W. N. Chen

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Hydroxyapatite based biomaterials were prepared by a spark plasma sintering technology. The human limb-derived osteoblasts were cultured on the various biomaterial surfaces (HA, RF21, 1SiHA and 5SiHA) for up to two weeks to investigate the cellular behaviors. The bone gammacarboxyglutamic protein or osteocalcin in the medium were determined at different periods of cell culture. The results indicated that a combined effect of bioceramic surface composition and surface morphology had influenced the osteoblast behaviors. The amount of osteocalcin in the medium increased in the initial periods of culture but decreased in the late periods of culture.

Original language	English
Pages (from-to)	897-900
Number of pages	4
Journal	Key Engineering Materials
Volume	330-332 II
DOIs	https://doi.org/10.4028/0-87849-422-7.897
Publication status	Published - 2007
Externally published	Yes

ASJC Scopus Subject Areas

General Materials Science
Mechanics of Materials
Mechanical Engineering

Keywords

Hydroxyapatite
In vitro
Osteocalcin
Spark plasma sintering

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10.4028/0-87849-422-7.897

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title = "A study of osteocalcin production in vitro on various hydroxyapatite based biomaterials",

abstract = "Hydroxyapatite based biomaterials were prepared by a spark plasma sintering technology. The human limb-derived osteoblasts were cultured on the various biomaterial surfaces (HA, RF21, 1SiHA and 5SiHA) for up to two weeks to investigate the cellular behaviors. The bone gammacarboxyglutamic protein or osteocalcin in the medium were determined at different periods of cell culture. The results indicated that a combined effect of bioceramic surface composition and surface morphology had influenced the osteoblast behaviors. The amount of osteocalcin in the medium increased in the initial periods of culture but decreased in the late periods of culture.",

keywords = "Hydroxyapatite, In vitro, Osteocalcin, Spark plasma sintering",

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T1 - A study of osteocalcin production in vitro on various hydroxyapatite based biomaterials

AU - Xu, J. L.

AU - Khor, K. A.

AU - Chen, W. N.

PY - 2007

Y1 - 2007

N2 - Hydroxyapatite based biomaterials were prepared by a spark plasma sintering technology. The human limb-derived osteoblasts were cultured on the various biomaterial surfaces (HA, RF21, 1SiHA and 5SiHA) for up to two weeks to investigate the cellular behaviors. The bone gammacarboxyglutamic protein or osteocalcin in the medium were determined at different periods of cell culture. The results indicated that a combined effect of bioceramic surface composition and surface morphology had influenced the osteoblast behaviors. The amount of osteocalcin in the medium increased in the initial periods of culture but decreased in the late periods of culture.

AB - Hydroxyapatite based biomaterials were prepared by a spark plasma sintering technology. The human limb-derived osteoblasts were cultured on the various biomaterial surfaces (HA, RF21, 1SiHA and 5SiHA) for up to two weeks to investigate the cellular behaviors. The bone gammacarboxyglutamic protein or osteocalcin in the medium were determined at different periods of cell culture. The results indicated that a combined effect of bioceramic surface composition and surface morphology had influenced the osteoblast behaviors. The amount of osteocalcin in the medium increased in the initial periods of culture but decreased in the late periods of culture.

KW - Hydroxyapatite

KW - In vitro

KW - Osteocalcin

KW - Spark plasma sintering

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JO - Key Engineering Materials

JF - Key Engineering Materials

ER -

A study of osteocalcin production in vitro on various hydroxyapatite based biomaterials

Abstract

ASJC Scopus Subject Areas

Keywords

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