Aerobic glycolysis is important for zebrafish larval wound closure and tail regeneration

Claire A. Scott, Tom J. Carney, Enrique Amaya*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The underlying mechanisms of appendage regeneration remain largely unknown and uncovering these mechanisms in capable organisms has far-reaching implications for potential treatments in humans. Recent studies implicate a requirement for metabolic reprogramming reminiscent of the Warburg effect during successful appendage and organ regeneration. As changes are thus predicted to be highly dynamic, methods permitting direct, real-time visualisation of metabolites at the tissue and organismal level would offer a significant advance in defining the influence of metabolism on regeneration and healing. We sought to examine whether glycolytic activity was altered during larval fin regeneration, utilising the genetically encoded biosensor, Laconic, enabling the spatiotemporal assessment of lactate levels in living zebrafish. We present evidence for a rapid increase in lactate levels within min following injury, with a role of aerobic glycolysis in actomyosin contraction and wound closure. We also find a second wave of lactate production, associated with overall larval tail regeneration. Chemical inhibition of glycolysis attenuates both the contraction of the wound and regrowth of tissue following tail amputation, suggesting aerobic glycolysis is necessary at two distinct stages of regeneration.

Original languageEnglish
Pages (from-to)665-680
Number of pages16
JournalWound Repair and Regeneration
Volume30
Issue number6
DOIs
Publication statusPublished - Nov 1 2022
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2022 The Authors. Wound Repair and Regeneration published by Wiley Periodicals LLC on behalf of The Wound Healing Society.

ASJC Scopus Subject Areas

  • Surgery
  • Dermatology

Keywords

  • appendage regeneration
  • laconic
  • lactate
  • metabolism
  • Warburg effect
  • wound healing

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