An optimized cyclophosphamide-treated mouse model of Mycobacterium abscessus pulmonary infection

Yan Sun; Kaixi Zhang; May Delos Santos; Carmen J.E. Pee; Yanmeng Yang; Meiqi Kang; Sung Jae Shin; Mary B. Chan-Park; Kevin Pethe

doi:10.1128/aac.01520-23

An optimized cyclophosphamide-treated mouse model of Mycobacterium abscessus pulmonary infection

Yan Sun, Kaixi Zhang, May Delos Santos, Carmen J.E. Pee, Yanmeng Yang, Meiqi Kang, Sung Jae Shin, Mary B. Chan-Park, Kevin Pethe^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Mycobacterium abscessus pulmonary infections are increasingly problematic, especially for immunocompromised individuals and those with underlying lung conditions. Currently, there is no reliable standardized treatment, underscoring the need for improved preclinical drug testing. We present a simplified immunosuppressed mouse model using only four injections of cyclophosphamide, which allows for sustained M. abscessus lung burden for up to 16 days. This model proved effective for antibiotic efficacy evaluation, as demonstrated with imipenem or amikacin.

Original language	English
Journal	Antimicrobial Agents and Chemotherapy
Volume	68
Issue number	8
DOIs	https://doi.org/10.1128/aac.01520-23
Publication status	Published - Aug 2024
Externally published	Yes

Bibliographical note

Publisher Copyright:
Copyright © 2024 American Society for Microbiology. All Rights Reserved.

ASJC Scopus Subject Areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

Keywords

lung infection
M. abscessus
mouse model

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/aac.01520-23

Cite this

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title = "An optimized cyclophosphamide-treated mouse model of Mycobacterium abscessus pulmonary infection",

abstract = "Mycobacterium abscessus pulmonary infections are increasingly problematic, especially for immunocompromised individuals and those with underlying lung conditions. Currently, there is no reliable standardized treatment, underscoring the need for improved preclinical drug testing. We present a simplified immunosuppressed mouse model using only four injections of cyclophosphamide, which allows for sustained M. abscessus lung burden for up to 16 days. This model proved effective for antibiotic efficacy evaluation, as demonstrated with imipenem or amikacin.",

keywords = "lung infection, M. abscessus, mouse model",

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year = "2024",

month = aug,

doi = "10.1128/aac.01520-23",

language = "English",

volume = "68",

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AU - Sun, Yan

AU - Zhang, Kaixi

AU - Santos, May Delos

AU - Pee, Carmen J.E.

AU - Yang, Yanmeng

AU - Kang, Meiqi

AU - Shin, Sung Jae

AU - Chan-Park, Mary B.

AU - Pethe, Kevin

PY - 2024/8

Y1 - 2024/8

N2 - Mycobacterium abscessus pulmonary infections are increasingly problematic, especially for immunocompromised individuals and those with underlying lung conditions. Currently, there is no reliable standardized treatment, underscoring the need for improved preclinical drug testing. We present a simplified immunosuppressed mouse model using only four injections of cyclophosphamide, which allows for sustained M. abscessus lung burden for up to 16 days. This model proved effective for antibiotic efficacy evaluation, as demonstrated with imipenem or amikacin.

AB - Mycobacterium abscessus pulmonary infections are increasingly problematic, especially for immunocompromised individuals and those with underlying lung conditions. Currently, there is no reliable standardized treatment, underscoring the need for improved preclinical drug testing. We present a simplified immunosuppressed mouse model using only four injections of cyclophosphamide, which allows for sustained M. abscessus lung burden for up to 16 days. This model proved effective for antibiotic efficacy evaluation, as demonstrated with imipenem or amikacin.

KW - lung infection

KW - M. abscessus

KW - mouse model

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UR - http://www.scopus.com/inward/citedby.url?scp=85200938507&partnerID=8YFLogxK

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M3 - Article

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AN - SCOPUS:85200938507

SN - 0066-4804

VL - 68

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 8

ER -

An optimized cyclophosphamide-treated mouse model of Mycobacterium abscessus pulmonary infection

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

UN SDGs

Access to Document

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