Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp

Silvanir; Sin Yuan Lai; Azren Aida Asmawi; Kit Wayne Chew; Cheng Loong Ngan

doi:10.1016/j.biortech.2023.130094

Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp

Silvanir, Sin Yuan Lai, Azren Aida Asmawi, Kit Wayne Chew, Cheng Loong Ngan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Microalgae is a sustainable alternative source to traditional proteins. Existing pretreatment methods for protein extraction from microalgae still lack scalability, are uneconomical and inefficient. Herein, high shear mixing (HSM) was applied to disrupt the rigid cell walls and was found to assist in protein release from microalgae. This study integrates HSM in liquid biphasic system with seven parameters being investigated on extraction efficiency (EE) and protein yield (Y). The highest EE and Y obtained are 96.83 ± 0.47 % and 40.98 ± 1.27 %, respectively, using 30% w/v K₃PO₄ salt, 60 % v/v alcohol, volume ratio of 1:1 and 0.5 % w/v biomass loading under shearing rate of 16,000 rpm for 1 min.

Original language	English
Article number	130094
Journal	Bioresource Technology
Volume	393
DOIs	https://doi.org/10.1016/j.biortech.2023.130094
Publication status	Published - Feb 2024
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2023 Elsevier Ltd

ASJC Scopus Subject Areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

Keywords

Aqueous two-phase system
Biorefinery
Chlorella vulgaris
Rotor-stator homogenization
Separation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2023.130094

Cite this

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title = "Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp",

abstract = "Microalgae is a sustainable alternative source to traditional proteins. Existing pretreatment methods for protein extraction from microalgae still lack scalability, are uneconomical and inefficient. Herein, high shear mixing (HSM) was applied to disrupt the rigid cell walls and was found to assist in protein release from microalgae. This study integrates HSM in liquid biphasic system with seven parameters being investigated on extraction efficiency (EE) and protein yield (Y). The highest EE and Y obtained are 96.83 ± 0.47 % and 40.98 ± 1.27 %, respectively, using 30% w/v K3PO4 salt, 60 % v/v alcohol, volume ratio of 1:1 and 0.5 % w/v biomass loading under shearing rate of 16,000 rpm for 1 min.",

keywords = "Aqueous two-phase system, Biorefinery, Chlorella vulgaris, Rotor-stator homogenization, Separation",

author = "Silvanir and Lai, {Sin Yuan} and Asmawi, {Azren Aida} and Chew, {Kit Wayne} and Ngan, {Cheng Loong}",

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year = "2024",

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doi = "10.1016/j.biortech.2023.130094",

language = "English",

volume = "393",

journal = "Bioresource Technology",

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T1 - Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp

AU - Silvanir,

AU - Lai, Sin Yuan

AU - Asmawi, Azren Aida

AU - Chew, Kit Wayne

AU - Ngan, Cheng Loong

PY - 2024/2

Y1 - 2024/2

N2 - Microalgae is a sustainable alternative source to traditional proteins. Existing pretreatment methods for protein extraction from microalgae still lack scalability, are uneconomical and inefficient. Herein, high shear mixing (HSM) was applied to disrupt the rigid cell walls and was found to assist in protein release from microalgae. This study integrates HSM in liquid biphasic system with seven parameters being investigated on extraction efficiency (EE) and protein yield (Y). The highest EE and Y obtained are 96.83 ± 0.47 % and 40.98 ± 1.27 %, respectively, using 30% w/v K3PO4 salt, 60 % v/v alcohol, volume ratio of 1:1 and 0.5 % w/v biomass loading under shearing rate of 16,000 rpm for 1 min.

AB - Microalgae is a sustainable alternative source to traditional proteins. Existing pretreatment methods for protein extraction from microalgae still lack scalability, are uneconomical and inefficient. Herein, high shear mixing (HSM) was applied to disrupt the rigid cell walls and was found to assist in protein release from microalgae. This study integrates HSM in liquid biphasic system with seven parameters being investigated on extraction efficiency (EE) and protein yield (Y). The highest EE and Y obtained are 96.83 ± 0.47 % and 40.98 ± 1.27 %, respectively, using 30% w/v K3PO4 salt, 60 % v/v alcohol, volume ratio of 1:1 and 0.5 % w/v biomass loading under shearing rate of 16,000 rpm for 1 min.

KW - Aqueous two-phase system

KW - Biorefinery

KW - Chlorella vulgaris

KW - Rotor-stator homogenization

KW - Separation

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Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

UN SDGs

Access to Document

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