Biochemical and functional characterization of the membrane association and membrane permeabilizing activity of the severe acute respiratory syndrome coronavirus envelope protein

Y. Liao, Q. Yuan, J. Torres, J. P. Tam, D. X. Liu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

117 Citations (Scopus)

Abstract

A diverse group of cytolytic animal viruses encodes small, hydrophobic proteins to modify host cell membrane permeability to ions and small molecules during their infection cycles. In this study, we show that expression of the SARS-CoV E protein in mammalian cells alters the membrane permeability of these cells. Immunofluorescent staining and cell fractionation studies demonstrate that this protein is an integral membrane protein. It is mainly localized to the ER and the Golgi apparatus. The protein can be translocated to the cell surface and is partially associated with lipid rafts. Further biochemical characterization of the protein reveals that it is posttranslationally modified by palmitoylation on all three cysteine residues. Systematic mutagenesis studies confirm that the membrane permeabilizing activity of the SARS-CoV E protein is associated with its transmembrane domain.

Original languageEnglish
Pages (from-to)264-275
Number of pages12
JournalVirology
Volume349
Issue number2
DOIs
Publication statusPublished - Jun 5 2006
Externally publishedYes

ASJC Scopus Subject Areas

  • Virology

Keywords

  • E protein
  • Membrane association
  • Palmitoylation
  • Permeabilizing activity
  • SARS-CoV

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