Catechol-modified green fluorescent protein as a specific biosensor for Al ions

Di Wu; Kanagavel Deepankumar; Yuan Ping; Guping Tang; Nadarajan Saravananprabhu; Ali Miserez; Wenjun Fang

doi:10.1016/j.snb.2017.05.021

Catechol-modified green fluorescent protein as a specific biosensor for Al ions

Di Wu, Kanagavel Deepankumar, Yuan Ping, Guping Tang, Nadarajan Saravananprabhu, Ali Miserez^*, Wenjun Fang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

A protein-based fluorescent sensor for the efficient detection of Al³⁺ is reported. The sensor is genetically engineered through the residue-specific incorporation of 3, 4-dihydroxy-L-phenylalanine (Dopa) into a green fluorescent protein (GFP) (termed as GFP-Dopa) and can selectively detect Al³⁺ in aqueous media. Al³⁺ could be readily detected through the distinctive fluorescence enhancement as a result of strong chelation of Al³⁺ with GFP-Dopa. Among 15 different tested metal ions, only Al³⁺ induces a significant fluorescence enhancement of GFP-Dopa, suggesting a high selectivity and exclusive specificity of GFP-Dopa towards Al³⁺. Together with its high biocompatibility, this protein-based sensor could be used in various applications requiring the rapid and sensitive detection of Al³⁺.

Original language	English
Pages (from-to)	326-333
Number of pages	8
Journal	Sensors and Actuators, B: Chemical
Volume	251
DOIs	https://doi.org/10.1016/j.snb.2017.05.021
Publication status	Published - 2017
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2017 Elsevier B.V.

ASJC Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

Keywords

Al
Dopa
Fluorescence enhancement
Protein biosensor
Residue-specific incorporation

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10.1016/j.snb.2017.05.021

Cite this

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title = "Catechol-modified green fluorescent protein as a specific biosensor for Al ions",

abstract = "A protein-based fluorescent sensor for the efficient detection of Al3+ is reported. The sensor is genetically engineered through the residue-specific incorporation of 3, 4-dihydroxy-L-phenylalanine (Dopa) into a green fluorescent protein (GFP) (termed as GFP-Dopa) and can selectively detect Al3+ in aqueous media. Al3+ could be readily detected through the distinctive fluorescence enhancement as a result of strong chelation of Al3+ with GFP-Dopa. Among 15 different tested metal ions, only Al3+ induces a significant fluorescence enhancement of GFP-Dopa, suggesting a high selectivity and exclusive specificity of GFP-Dopa towards Al3+. Together with its high biocompatibility, this protein-based sensor could be used in various applications requiring the rapid and sensitive detection of Al3+.",

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T1 - Catechol-modified green fluorescent protein as a specific biosensor for Al ions

AU - Wu, Di

AU - Deepankumar, Kanagavel

AU - Ping, Yuan

AU - Tang, Guping

AU - Saravananprabhu, Nadarajan

AU - Miserez, Ali

AU - Fang, Wenjun

PY - 2017

Y1 - 2017

N2 - A protein-based fluorescent sensor for the efficient detection of Al3+ is reported. The sensor is genetically engineered through the residue-specific incorporation of 3, 4-dihydroxy-L-phenylalanine (Dopa) into a green fluorescent protein (GFP) (termed as GFP-Dopa) and can selectively detect Al3+ in aqueous media. Al3+ could be readily detected through the distinctive fluorescence enhancement as a result of strong chelation of Al3+ with GFP-Dopa. Among 15 different tested metal ions, only Al3+ induces a significant fluorescence enhancement of GFP-Dopa, suggesting a high selectivity and exclusive specificity of GFP-Dopa towards Al3+. Together with its high biocompatibility, this protein-based sensor could be used in various applications requiring the rapid and sensitive detection of Al3+.

AB - A protein-based fluorescent sensor for the efficient detection of Al3+ is reported. The sensor is genetically engineered through the residue-specific incorporation of 3, 4-dihydroxy-L-phenylalanine (Dopa) into a green fluorescent protein (GFP) (termed as GFP-Dopa) and can selectively detect Al3+ in aqueous media. Al3+ could be readily detected through the distinctive fluorescence enhancement as a result of strong chelation of Al3+ with GFP-Dopa. Among 15 different tested metal ions, only Al3+ induces a significant fluorescence enhancement of GFP-Dopa, suggesting a high selectivity and exclusive specificity of GFP-Dopa towards Al3+. Together with its high biocompatibility, this protein-based sensor could be used in various applications requiring the rapid and sensitive detection of Al3+.

KW - Al

KW - Dopa

KW - Fluorescence enhancement

KW - Protein biosensor

KW - Residue-specific incorporation

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ER -

Catechol-modified green fluorescent protein as a specific biosensor for Al ions

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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