Cloning and expression of a Bacillus licheniformis α-amylase gene in Pseudomonas aeruginosa

A. Filloux; P. Joyet; M. Murgier; A. Lazdunski

doi:10.1111/j.1574-6968.1985.tb01012.x

Cloning and expression of a Bacillus licheniformis α-amylase gene in Pseudomonas aeruginosa

A. Filloux^*, P. Joyet, M. Murgier, A. Lazdunski

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The gene for B. licheniformis α-amylase has been cloned in P. aeruginosa. Synthesis of the enzyme occurs in late log phase and goes on during stationary phase. Although P. aeruginosa is a secretory bacterium, α-amylase is not efficiently secreted into the extracellular medium; 85% of the enzyme is retained in the periplasm.

Original language	English
Pages (from-to)	203-207
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	30
Issue number	1-2
DOIs	https://doi.org/10.1111/j.1574-6968.1985.tb01012.x
Publication status	Published - 1985
Externally published	Yes

ASJC Scopus Subject Areas

Microbiology
Molecular Biology
Genetics

Keywords

B. licheniformis α-amylase
Pseudomonas
Secretion of foreign protein

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10.1111/j.1574-6968.1985.tb01012.x

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title = "Cloning and expression of a Bacillus licheniformis α-amylase gene in Pseudomonas aeruginosa",

abstract = "The gene for B. licheniformis α-amylase has been cloned in P. aeruginosa. Synthesis of the enzyme occurs in late log phase and goes on during stationary phase. Although P. aeruginosa is a secretory bacterium, α-amylase is not efficiently secreted into the extracellular medium; 85% of the enzyme is retained in the periplasm.",

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author = "A. Filloux and P. Joyet and M. Murgier and A. Lazdunski",

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AU - Filloux, A.

AU - Joyet, P.

AU - Murgier, M.

AU - Lazdunski, A.

PY - 1985

Y1 - 1985

N2 - The gene for B. licheniformis α-amylase has been cloned in P. aeruginosa. Synthesis of the enzyme occurs in late log phase and goes on during stationary phase. Although P. aeruginosa is a secretory bacterium, α-amylase is not efficiently secreted into the extracellular medium; 85% of the enzyme is retained in the periplasm.

AB - The gene for B. licheniformis α-amylase has been cloned in P. aeruginosa. Synthesis of the enzyme occurs in late log phase and goes on during stationary phase. Although P. aeruginosa is a secretory bacterium, α-amylase is not efficiently secreted into the extracellular medium; 85% of the enzyme is retained in the periplasm.

KW - B. licheniformis α-amylase

KW - Pseudomonas

KW - Secretion of foreign protein

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U2 - 10.1111/j.1574-6968.1985.tb01012.x

DO - 10.1111/j.1574-6968.1985.tb01012.x

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JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1-2

ER -

Cloning and expression of a Bacillus licheniformis α-amylase gene in Pseudomonas aeruginosa

Abstract

ASJC Scopus Subject Areas

Keywords

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