Colorimetric Dopamine Detection Based on Immobilized Monoamine Oxidase through Silver Nanoparticles

Gounhanul Shin; Kunn Hadinoto; Jin Won Park

doi:10.18494/SAM4356

Colorimetric Dopamine Detection Based on Immobilized Monoamine Oxidase through Silver Nanoparticles

Gounhanul Shin, Kunn Hadinoto, Jin Won Park^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The colorimetric detection of dopamine was investigated to enhance the dopamine detection sensitivity. The detection was through the oxidation of AgNPs by hydrogen peroxide converted from dopamine along with dihydroxyphenylacetaldehyde. The conversion was facilitated by monoamine oxidase-Bs (MAO-Bs) bound to biomimetic membranes. The absorbance at 410 nm decreased with the increase in dopamine concentration. The limit of detection was determined to be 0.013 μM for 0.01 to 3.5 μM dopamine in the linear range of absorbance. The absorbance change corresponded to the conversion of Ag to Ag₂O, which occurred with the hydrogen peroxide released through MAO-Bs from dopamine. These results indicate that the AgNP degradation based on the immobilized MAO-Bs is sufficiently appropriate for the high-sensitivity absorbance detection of dopamine with a wide linear range.

Original language	English
Pages (from-to)	1767-1773
Number of pages	7
Journal	Sensors and Materials
Volume	35
Issue number	5
DOIs	https://doi.org/10.18494/SAM4356
Publication status	Published - 2023
Externally published	Yes

Bibliographical note

Publisher Copyright:
© MYU K.K.

ASJC Scopus Subject Areas

Instrumentation
General Materials Science

Keywords

dopamine
monoamine oxidase
oxidation
silver nanoparticles

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10.18494/SAM4356

Cite this

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title = "Colorimetric Dopamine Detection Based on Immobilized Monoamine Oxidase through Silver Nanoparticles",

abstract = "The colorimetric detection of dopamine was investigated to enhance the dopamine detection sensitivity. The detection was through the oxidation of AgNPs by hydrogen peroxide converted from dopamine along with dihydroxyphenylacetaldehyde. The conversion was facilitated by monoamine oxidase-Bs (MAO-Bs) bound to biomimetic membranes. The absorbance at 410 nm decreased with the increase in dopamine concentration. The limit of detection was determined to be 0.013 μM for 0.01 to 3.5 μM dopamine in the linear range of absorbance. The absorbance change corresponded to the conversion of Ag to Ag2O, which occurred with the hydrogen peroxide released through MAO-Bs from dopamine. These results indicate that the AgNP degradation based on the immobilized MAO-Bs is sufficiently appropriate for the high-sensitivity absorbance detection of dopamine with a wide linear range.",

keywords = "dopamine, monoamine oxidase, oxidation, silver nanoparticles",

author = "Gounhanul Shin and Kunn Hadinoto and Park, {Jin Won}",

note = "Publisher Copyright: {\textcopyright} MYU K.K.",

year = "2023",

doi = "10.18494/SAM4356",

language = "English",

volume = "35",

pages = "1767--1773",

journal = "Sensors and Materials",

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}

TY - JOUR

T1 - Colorimetric Dopamine Detection Based on Immobilized Monoamine Oxidase through Silver Nanoparticles

AU - Shin, Gounhanul

AU - Hadinoto, Kunn

AU - Park, Jin Won

N1 - Publisher Copyright: © MYU K.K.

PY - 2023

Y1 - 2023

N2 - The colorimetric detection of dopamine was investigated to enhance the dopamine detection sensitivity. The detection was through the oxidation of AgNPs by hydrogen peroxide converted from dopamine along with dihydroxyphenylacetaldehyde. The conversion was facilitated by monoamine oxidase-Bs (MAO-Bs) bound to biomimetic membranes. The absorbance at 410 nm decreased with the increase in dopamine concentration. The limit of detection was determined to be 0.013 μM for 0.01 to 3.5 μM dopamine in the linear range of absorbance. The absorbance change corresponded to the conversion of Ag to Ag2O, which occurred with the hydrogen peroxide released through MAO-Bs from dopamine. These results indicate that the AgNP degradation based on the immobilized MAO-Bs is sufficiently appropriate for the high-sensitivity absorbance detection of dopamine with a wide linear range.

AB - The colorimetric detection of dopamine was investigated to enhance the dopamine detection sensitivity. The detection was through the oxidation of AgNPs by hydrogen peroxide converted from dopamine along with dihydroxyphenylacetaldehyde. The conversion was facilitated by monoamine oxidase-Bs (MAO-Bs) bound to biomimetic membranes. The absorbance at 410 nm decreased with the increase in dopamine concentration. The limit of detection was determined to be 0.013 μM for 0.01 to 3.5 μM dopamine in the linear range of absorbance. The absorbance change corresponded to the conversion of Ag to Ag2O, which occurred with the hydrogen peroxide released through MAO-Bs from dopamine. These results indicate that the AgNP degradation based on the immobilized MAO-Bs is sufficiently appropriate for the high-sensitivity absorbance detection of dopamine with a wide linear range.

KW - dopamine

KW - monoamine oxidase

KW - oxidation

KW - silver nanoparticles

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DO - 10.18494/SAM4356

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SN - 0914-4935

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SP - 1767

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JO - Sensors and Materials

JF - Sensors and Materials

IS - 5

ER -

Colorimetric Dopamine Detection Based on Immobilized Monoamine Oxidase through Silver Nanoparticles

Abstract

Bibliographical note

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Keywords

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