Colorimetric microneedle patches for multiplexed transdermal detection of metabolites

Dan Dan Zhu; Le Wen Zheng; Phan Khanh Duong; Reanne Hui Cheah; Xin Yi Liu; Jun Ren Wong; Wen Jun Wang; Steven Thng Tien Guan; Xin Ting Zheng; Peng Chen

doi:10.1016/j.bios.2022.114412

Colorimetric microneedle patches for multiplexed transdermal detection of metabolites

Dan Dan Zhu, Le Wen Zheng, Phan Khanh Duong, Reanne Hui Cheah, Xin Yi Liu, Jun Ren Wong, Wen Jun Wang, Steven Thng Tien Guan, Xin Ting Zheng^*, Peng Chen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

Abstract

Skin Interstitial Fluid (ISF) is an alternative source for biomarkers. Herein, a highly swellable microneedle patch (MNP) to rapidly extract ISF painlessly and bloodlessly is presented. The MNP is made of crosslinked methacrylated hyaluronic acid (MeHA) and dissolvable hyaluronic acid (HA) with the optimal balance of mechanical strength (0.6 N/MN) and absorption capability (16.22 μL in 20 min). Incorporated with wax-patterned and sensing-reagent-decorated test paper (TP) for multiplexed colorimetric detection of metabolites (glucose, lactate, cholesterol, and pH), this TP-MNP biosensor gives rapid color change in biomarker concentration-dependent manner based on specific enzymatic reactions, whereby allowing diagnosis by the naked eye or quantitative RGB analysis. Both the in vitro and in vivo experiments demonstrate the feasibility of TP-MNPs to detect multiple biomarkers in skin interstitial fluid within minutes. Such convenient and self-administrable profiling of metabolites shall be instrumental for home-based long-term monitoring and management of metabolic diseases.

Original language	English
Article number	114412
Journal	Biosensors and Bioelectronics
Volume	212
DOIs	https://doi.org/10.1016/j.bios.2022.114412
Publication status	Published - Sept 15 2022
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2022 Elsevier B.V.

ASJC Scopus Subject Areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

Keywords

Metabolites
Microneedles
Skin interstitial fluid
Transdermal detection

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10.1016/j.bios.2022.114412

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title = "Colorimetric microneedle patches for multiplexed transdermal detection of metabolites",

abstract = "Skin Interstitial Fluid (ISF) is an alternative source for biomarkers. Herein, a highly swellable microneedle patch (MNP) to rapidly extract ISF painlessly and bloodlessly is presented. The MNP is made of crosslinked methacrylated hyaluronic acid (MeHA) and dissolvable hyaluronic acid (HA) with the optimal balance of mechanical strength (0.6 N/MN) and absorption capability (16.22 μL in 20 min). Incorporated with wax-patterned and sensing-reagent-decorated test paper (TP) for multiplexed colorimetric detection of metabolites (glucose, lactate, cholesterol, and pH), this TP-MNP biosensor gives rapid color change in biomarker concentration-dependent manner based on specific enzymatic reactions, whereby allowing diagnosis by the naked eye or quantitative RGB analysis. Both the in vitro and in vivo experiments demonstrate the feasibility of TP-MNPs to detect multiple biomarkers in skin interstitial fluid within minutes. Such convenient and self-administrable profiling of metabolites shall be instrumental for home-based long-term monitoring and management of metabolic diseases.",

keywords = "Metabolites, Microneedles, Skin interstitial fluid, Transdermal detection",

author = "Zhu, {Dan Dan} and Zheng, {Le Wen} and Duong, {Phan Khanh} and Cheah, {Reanne Hui} and Liu, {Xin Yi} and Wong, {Jun Ren} and Wang, {Wen Jun} and {Tien Guan}, {Steven Thng} and Zheng, {Xin Ting} and Peng Chen",

note = "Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

year = "2022",

month = sep,

day = "15",

doi = "10.1016/j.bios.2022.114412",

language = "English",

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T1 - Colorimetric microneedle patches for multiplexed transdermal detection of metabolites

AU - Zhu, Dan Dan

AU - Zheng, Le Wen

AU - Duong, Phan Khanh

AU - Cheah, Reanne Hui

AU - Liu, Xin Yi

AU - Wong, Jun Ren

AU - Wang, Wen Jun

AU - Tien Guan, Steven Thng

AU - Zheng, Xin Ting

AU - Chen, Peng

PY - 2022/9/15

Y1 - 2022/9/15

N2 - Skin Interstitial Fluid (ISF) is an alternative source for biomarkers. Herein, a highly swellable microneedle patch (MNP) to rapidly extract ISF painlessly and bloodlessly is presented. The MNP is made of crosslinked methacrylated hyaluronic acid (MeHA) and dissolvable hyaluronic acid (HA) with the optimal balance of mechanical strength (0.6 N/MN) and absorption capability (16.22 μL in 20 min). Incorporated with wax-patterned and sensing-reagent-decorated test paper (TP) for multiplexed colorimetric detection of metabolites (glucose, lactate, cholesterol, and pH), this TP-MNP biosensor gives rapid color change in biomarker concentration-dependent manner based on specific enzymatic reactions, whereby allowing diagnosis by the naked eye or quantitative RGB analysis. Both the in vitro and in vivo experiments demonstrate the feasibility of TP-MNPs to detect multiple biomarkers in skin interstitial fluid within minutes. Such convenient and self-administrable profiling of metabolites shall be instrumental for home-based long-term monitoring and management of metabolic diseases.

AB - Skin Interstitial Fluid (ISF) is an alternative source for biomarkers. Herein, a highly swellable microneedle patch (MNP) to rapidly extract ISF painlessly and bloodlessly is presented. The MNP is made of crosslinked methacrylated hyaluronic acid (MeHA) and dissolvable hyaluronic acid (HA) with the optimal balance of mechanical strength (0.6 N/MN) and absorption capability (16.22 μL in 20 min). Incorporated with wax-patterned and sensing-reagent-decorated test paper (TP) for multiplexed colorimetric detection of metabolites (glucose, lactate, cholesterol, and pH), this TP-MNP biosensor gives rapid color change in biomarker concentration-dependent manner based on specific enzymatic reactions, whereby allowing diagnosis by the naked eye or quantitative RGB analysis. Both the in vitro and in vivo experiments demonstrate the feasibility of TP-MNPs to detect multiple biomarkers in skin interstitial fluid within minutes. Such convenient and self-administrable profiling of metabolites shall be instrumental for home-based long-term monitoring and management of metabolic diseases.

KW - Metabolites

KW - Microneedles

KW - Skin interstitial fluid

KW - Transdermal detection

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Colorimetric microneedle patches for multiplexed transdermal detection of metabolites

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

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