Continuous flow microfluidic system for customizable bacterial cellulose production through synthetic biology

Jiaqing Yu, Guoyun Sun, Myat Noe Hsu, Vishnu Vadanan Sundaravadanam, Sierin Lim, Chia Hung Chen*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper presents a high-throughput continuous-flow microfluidic sorting system for customizable bacterial cellulose (BC) production. Gluconacetobacter xylinus was encapsulated in agarose hydrogel particle using droplet based microfluidics for a long-time static culture to generate BC. A real time image analysis system was then applied to distinguish the hydrogel particles according to their BC content by the light scattering pattern. This sorting device could select the target hydrogel particles containing bacteria capable of producing high BC volume for downstream genome analysis.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages961-962
Number of pages2
ISBN (Electronic)9780692941836
Publication statusPublished - 2020
Externally publishedYes
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: Oct 22 2017Oct 26 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Country/TerritoryUnited States
CitySavannah
Period10/22/1710/26/17

Bibliographical note

Publisher Copyright:
© 17CBMS-0001.

ASJC Scopus Subject Areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Keywords

  • Bacterial cellulose
  • Hydrogel
  • Image sorting
  • Microfluidic
  • Synthetic biology

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