Contribution of cyclic di-GMP in the control of type III and type VI secretion in pseudomonas aeruginosa

Ronan R. McCarthy; Martina Valentini; Alain Filloux

doi:10.1007/978-1-4939-7240-1_17

Contribution of cyclic di-GMP in the control of type III and type VI secretion in pseudomonas aeruginosa

Ronan R. McCarthy, Martina Valentini, Alain Filloux^*

^*Corresponding author for this work

Imperial College London

Research output: Chapter in Book/Report/Conference proceeding › Chapter

13 Citations (Scopus)

Abstract

Bacteria produce toxins to enhance their competitiveness in the colonization of an environment as well as during an infection. The delivery of toxins into target cells is mediated by several types of secretion systems, among them our focus is Type III and Type VI Secretion Systems (T3SS and T6SS, respectively). A thorough methodology is provided detailing how to identify if cyclic di-GMP signaling plays a role in the P. aeruginosa toxin delivery mediated by T3SS or T6SS. This includes in vitro preparation of the samples for Western blot analysis aiming at detecting possible c-di-GMP-dependent T3SS/T6SS switch, as well as in vivo analysis using the model organism Galleria mellonella to demonstrate the ecological and pathogenic consequence of the switch between these two secretion systems.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	213-224
Number of pages	12
DOIs	https://doi.org/10.1007/978-1-4939-7240-1_17
Publication status	Published - 2017
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1657
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media LLC 2017.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

Keywords

Bacterial toxins
Cyclic di-GMP
Galleria mellonella
Pseudomonas aeruginosa
Type III secretion
Type VI secretion

Access to Document

10.1007/978-1-4939-7240-1_17

Cite this

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title = "Contribution of cyclic di-GMP in the control of type III and type VI secretion in pseudomonas aeruginosa",

abstract = "Bacteria produce toxins to enhance their competitiveness in the colonization of an environment as well as during an infection. The delivery of toxins into target cells is mediated by several types of secretion systems, among them our focus is Type III and Type VI Secretion Systems (T3SS and T6SS, respectively). A thorough methodology is provided detailing how to identify if cyclic di-GMP signaling plays a role in the P. aeruginosa toxin delivery mediated by T3SS or T6SS. This includes in vitro preparation of the samples for Western blot analysis aiming at detecting possible c-di-GMP-dependent T3SS/T6SS switch, as well as in vivo analysis using the model organism Galleria mellonella to demonstrate the ecological and pathogenic consequence of the switch between these two secretion systems.",

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AU - Valentini, Martina

AU - Filloux, Alain

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PY - 2017

Y1 - 2017

N2 - Bacteria produce toxins to enhance their competitiveness in the colonization of an environment as well as during an infection. The delivery of toxins into target cells is mediated by several types of secretion systems, among them our focus is Type III and Type VI Secretion Systems (T3SS and T6SS, respectively). A thorough methodology is provided detailing how to identify if cyclic di-GMP signaling plays a role in the P. aeruginosa toxin delivery mediated by T3SS or T6SS. This includes in vitro preparation of the samples for Western blot analysis aiming at detecting possible c-di-GMP-dependent T3SS/T6SS switch, as well as in vivo analysis using the model organism Galleria mellonella to demonstrate the ecological and pathogenic consequence of the switch between these two secretion systems.

AB - Bacteria produce toxins to enhance their competitiveness in the colonization of an environment as well as during an infection. The delivery of toxins into target cells is mediated by several types of secretion systems, among them our focus is Type III and Type VI Secretion Systems (T3SS and T6SS, respectively). A thorough methodology is provided detailing how to identify if cyclic di-GMP signaling plays a role in the P. aeruginosa toxin delivery mediated by T3SS or T6SS. This includes in vitro preparation of the samples for Western blot analysis aiming at detecting possible c-di-GMP-dependent T3SS/T6SS switch, as well as in vivo analysis using the model organism Galleria mellonella to demonstrate the ecological and pathogenic consequence of the switch between these two secretion systems.

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KW - Pseudomonas aeruginosa

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KW - Type VI secretion

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Contribution of cyclic di-GMP in the control of type III and type VI secretion in pseudomonas aeruginosa

Abstract

Publication series

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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