Crystal structure of subunits D and F in complex gives insight into energy transmission of the eukaryotic V-ATPase from Saccharomyces cerevisiae

Asha Manikkoth Balakrishna; Sandip Basak; Malathy Sony Subramanian Manimekalai; Gerhard Grüber

doi:10.1074/jbc.M114.622688

Crystal structure of subunits D and F in complex gives insight into energy transmission of the eukaryotic V-ATPase from Saccharomyces cerevisiae

Asha Manikkoth Balakrishna, Sandip Basak, Malathy Sony Subramanian Manimekalai, Gerhard Grüber^*

^*Corresponding author for this work

Nanyang Technological University

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Background: Subunits D and F are essential in ATP hydrolysis and reversible disassembly of V₁V_O-ATPases. Results: First crystallographic structure of eukaryotic V-ATPase subunit D and entire subunit F is presented. Conclusion: Structural elements in subunits D and F enable the DF assembly to become a central motor element in the engine V-ATPase. Significance: Mechanistic understanding of the subunit DF assembly and its key roles in enzyme catalysis and regulation are presented.

Original language	English
Pages (from-to)	3183-3196
Number of pages	14
Journal	Journal of Biological Chemistry
Volume	290
Issue number	6
DOIs	https://doi.org/10.1074/jbc.M114.622688
Publication status	Published - Feb 6 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

ASJC Scopus Subject Areas

Biochemistry
Molecular Biology
Cell Biology

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10.1074/jbc.M114.622688

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abstract = "Background: Subunits D and F are essential in ATP hydrolysis and reversible disassembly of V1VO-ATPases. Results: First crystallographic structure of eukaryotic V-ATPase subunit D and entire subunit F is presented. Conclusion: Structural elements in subunits D and F enable the DF assembly to become a central motor element in the engine V-ATPase. Significance: Mechanistic understanding of the subunit DF assembly and its key roles in enzyme catalysis and regulation are presented.",

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Crystal structure of subunits D and F in complex gives insight into energy transmission of the eukaryotic V-ATPase from Saccharomyces cerevisiae. / Balakrishna, Asha Manikkoth; Basak, Sandip; Manimekalai, Malathy Sony Subramanian et al.
In: Journal of Biological Chemistry, Vol. 290, No. 6, 06.02.2015, p. 3183-3196.

Research output: Contribution to journal › Article › peer-review

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AU - Balakrishna, Asha Manikkoth

AU - Basak, Sandip

AU - Manimekalai, Malathy Sony Subramanian

AU - Grüber, Gerhard

PY - 2015/2/6

Y1 - 2015/2/6

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AB - Background: Subunits D and F are essential in ATP hydrolysis and reversible disassembly of V1VO-ATPases. Results: First crystallographic structure of eukaryotic V-ATPase subunit D and entire subunit F is presented. Conclusion: Structural elements in subunits D and F enable the DF assembly to become a central motor element in the engine V-ATPase. Significance: Mechanistic understanding of the subunit DF assembly and its key roles in enzyme catalysis and regulation are presented.

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Crystal structure of subunits D and F in complex gives insight into energy transmission of the eukaryotic V-ATPase from Saccharomyces cerevisiae

Abstract

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