Cytochrome c oxidase rapidly metabolises nitric oxide to nitrite

Jaume Torres; Martyn A. Sharpe; Aasa Rosquist; Chris E. Cooper; Mike T. Wilson

doi:10.1016/S0014-5793(00)01682-3

Cytochrome c oxidase rapidly metabolises nitric oxide to nitrite

Jaume Torres, Martyn A. Sharpe, Aasa Rosquist, Chris E. Cooper, Mike T. Wilson^*

^*Corresponding author for this work

University of Essex

Research output: Contribution to journal › Article › peer-review

106 Citations (Scopus)

Abstract

Previous studies have shown that the addition of nitric oxide to cytochrome c oxidase rapidly generates spectral changes compatible with the formation of nitrite at the binuclear haem:copper centre. Here we directly demonstrate nitrite release following nitric oxide addition to the enzyme. The nitrite complex is kinetically inactive and the off rate for nitrite was found to be slow (0.024 min^-1). However, the presence of reductants enhances the off rate and enables cytochrome oxidase to catalyse the rapid oxidation of nitric oxide to nitrite free in solution. This may play a major role in the mitochondrial metabolism of nitric oxide. (C) 2000 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	263-266
Number of pages	4
Journal	FEBS Letters
Volume	475
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(00)01682-3
Publication status	Published - Jun 23 2000
Externally published	Yes

ASJC Scopus Subject Areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Keywords

Cytochrome c
Cytochrome c oxidase
Inhibition
Metabolism
Nitric oxide
Nitrite

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10.1016/S0014-5793(00)01682-3

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title = "Cytochrome c oxidase rapidly metabolises nitric oxide to nitrite",

abstract = "Previous studies have shown that the addition of nitric oxide to cytochrome c oxidase rapidly generates spectral changes compatible with the formation of nitrite at the binuclear haem:copper centre. Here we directly demonstrate nitrite release following nitric oxide addition to the enzyme. The nitrite complex is kinetically inactive and the off rate for nitrite was found to be slow (0.024 min-1). However, the presence of reductants enhances the off rate and enables cytochrome oxidase to catalyse the rapid oxidation of nitric oxide to nitrite free in solution. This may play a major role in the mitochondrial metabolism of nitric oxide. (C) 2000 Federation of European Biochemical Societies.",

keywords = "Cytochrome c, Cytochrome c oxidase, Inhibition, Metabolism, Nitric oxide, Nitrite",

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T1 - Cytochrome c oxidase rapidly metabolises nitric oxide to nitrite

AU - Torres, Jaume

AU - Sharpe, Martyn A.

AU - Rosquist, Aasa

AU - Cooper, Chris E.

AU - Wilson, Mike T.

PY - 2000/6/23

Y1 - 2000/6/23

N2 - Previous studies have shown that the addition of nitric oxide to cytochrome c oxidase rapidly generates spectral changes compatible with the formation of nitrite at the binuclear haem:copper centre. Here we directly demonstrate nitrite release following nitric oxide addition to the enzyme. The nitrite complex is kinetically inactive and the off rate for nitrite was found to be slow (0.024 min-1). However, the presence of reductants enhances the off rate and enables cytochrome oxidase to catalyse the rapid oxidation of nitric oxide to nitrite free in solution. This may play a major role in the mitochondrial metabolism of nitric oxide. (C) 2000 Federation of European Biochemical Societies.

AB - Previous studies have shown that the addition of nitric oxide to cytochrome c oxidase rapidly generates spectral changes compatible with the formation of nitrite at the binuclear haem:copper centre. Here we directly demonstrate nitrite release following nitric oxide addition to the enzyme. The nitrite complex is kinetically inactive and the off rate for nitrite was found to be slow (0.024 min-1). However, the presence of reductants enhances the off rate and enables cytochrome oxidase to catalyse the rapid oxidation of nitric oxide to nitrite free in solution. This may play a major role in the mitochondrial metabolism of nitric oxide. (C) 2000 Federation of European Biochemical Societies.

KW - Cytochrome c

KW - Cytochrome c oxidase

KW - Inhibition

KW - Metabolism

KW - Nitric oxide

KW - Nitrite

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U2 - 10.1016/S0014-5793(00)01682-3

DO - 10.1016/S0014-5793(00)01682-3

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VL - 475

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JO - FEBS Letters

JF - FEBS Letters

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ER -

Cytochrome c oxidase rapidly metabolises nitric oxide to nitrite

Abstract

ASJC Scopus Subject Areas

Keywords

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