Design of a pH-dependent molecular switch in a caged protein platform

Mercè Dalmau; Sierin Lim; Szu Wen Wang

doi:10.1021/nl8027069

Design of a pH-dependent molecular switch in a caged protein platform

Mercè Dalmau, Sierin Lim, Szu Wen Wang

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

Self-assembling protein cages provide a wide range of possible applications in nanotechnology. We report the first example of an engineered pH-dependent molecular switch in a virus-like particle. By genetically manipulating the subunit-subunit interface of the E2 subunit of pyruvate dehydrogenase, we introduce pH-responsive assembly into a scaffold that is natively stable at both pH 5.0 and 7.4. The redesigned protein module yields an intact, stable particle at pH 7.4 that dissociates at pH 5.0. This triggered behavior is especially relevant for applications in therapeutic delivery.

Original language	English
Pages (from-to)	160-166
Number of pages	7
Journal	Nano Letters
Volume	9
Issue number	1
DOIs	https://doi.org/10.1021/nl8027069
Publication status	Published - Jan 2009
Externally published	Yes

ASJC Scopus Subject Areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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10.1021/nl8027069

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Design of a pH-dependent molecular switch in a caged protein platform

Abstract

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