Detection and survival of pathogens during two-stage thermophilic/mesophilic anaerobic treatment of suspended organic waste

C. Burtscher; P. A. Fall; O. Christ; P. A. Wilderer; S. Wuertz

doi:10.2166/wst.1998.0520

Detection and survival of pathogens during two-stage thermophilic/mesophilic anaerobic treatment of suspended organic waste

C. Burtscher^*, P. A. Fall, O. Christ, P. A. Wilderer, S. Wuertz

^*Corresponding author for this work

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

Abstract

The food-borne pathogens Salmonella typhimurium and Listeria monocytogenes were effectively eliminated from suspended organic waste during the thermophilic (55°C) phase of a two-stage thermophilic/mesophilic anaerobic reactor. The feasibility of PCR technology was evaluated in order to improve the speed of detection and reduce the effort involved in detecting the presence of a range of pathogenic bacteria. Our results show direct extraction of nucleic acids followed by purification and PCR to be a promising method that will enable the rapid verification of hygienic performance during waste treatment.

Original language	English
Pages (from-to)	123-126
Number of pages	4
Journal	Water Science and Technology
Volume	38
Issue number	12
DOIs	https://doi.org/10.2166/wst.1998.0520
Publication status	Published - 1998
Externally published	Yes

ASJC Scopus Subject Areas

Environmental Engineering
Water Science and Technology

Keywords

Anaerobic digestion
Hygienic performance
Nucleic acid extraction
Organic waste
Pathogens
Polymerase chain reaction

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10.2166/wst.1998.0520

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title = "Detection and survival of pathogens during two-stage thermophilic/mesophilic anaerobic treatment of suspended organic waste",

abstract = "The food-borne pathogens Salmonella typhimurium and Listeria monocytogenes were effectively eliminated from suspended organic waste during the thermophilic (55°C) phase of a two-stage thermophilic/mesophilic anaerobic reactor. The feasibility of PCR technology was evaluated in order to improve the speed of detection and reduce the effort involved in detecting the presence of a range of pathogenic bacteria. Our results show direct extraction of nucleic acids followed by purification and PCR to be a promising method that will enable the rapid verification of hygienic performance during waste treatment.",

keywords = "Anaerobic digestion, Hygienic performance, Nucleic acid extraction, Organic waste, Pathogens, Polymerase chain reaction",

author = "C. Burtscher and Fall, {P. A.} and O. Christ and Wilderer, {P. A.} and S. Wuertz",

year = "1998",

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language = "English",

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pages = "123--126",

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T1 - Detection and survival of pathogens during two-stage thermophilic/mesophilic anaerobic treatment of suspended organic waste

AU - Burtscher, C.

AU - Fall, P. A.

AU - Christ, O.

AU - Wilderer, P. A.

AU - Wuertz, S.

PY - 1998

Y1 - 1998

N2 - The food-borne pathogens Salmonella typhimurium and Listeria monocytogenes were effectively eliminated from suspended organic waste during the thermophilic (55°C) phase of a two-stage thermophilic/mesophilic anaerobic reactor. The feasibility of PCR technology was evaluated in order to improve the speed of detection and reduce the effort involved in detecting the presence of a range of pathogenic bacteria. Our results show direct extraction of nucleic acids followed by purification and PCR to be a promising method that will enable the rapid verification of hygienic performance during waste treatment.

AB - The food-borne pathogens Salmonella typhimurium and Listeria monocytogenes were effectively eliminated from suspended organic waste during the thermophilic (55°C) phase of a two-stage thermophilic/mesophilic anaerobic reactor. The feasibility of PCR technology was evaluated in order to improve the speed of detection and reduce the effort involved in detecting the presence of a range of pathogenic bacteria. Our results show direct extraction of nucleic acids followed by purification and PCR to be a promising method that will enable the rapid verification of hygienic performance during waste treatment.

KW - Anaerobic digestion

KW - Hygienic performance

KW - Nucleic acid extraction

KW - Organic waste

KW - Pathogens

KW - Polymerase chain reaction

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DO - 10.2166/wst.1998.0520

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JO - Water Science and Technology

JF - Water Science and Technology

IS - 12

ER -

Detection and survival of pathogens during two-stage thermophilic/mesophilic anaerobic treatment of suspended organic waste

Abstract

ASJC Scopus Subject Areas

Keywords

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