Detection of high-level tetracycline resistance in clinical isolates of Helicobacter pylori using PCR-RFLP

Marcelo L. Ribeiro; Monique M. Gerrits; Yune H.B. Benvengo; Marco Berning; Anita P.O. Godoy; Ernst J. Kuipers; Sergio Mendonça; Arnoud H.M. Van Vliet; José Pedrazzoli; Johannes G. Kusters

doi:10.1016/S0928-8244(03)00277-3

Detection of high-level tetracycline resistance in clinical isolates of Helicobacter pylori using PCR-RFLP

Marcelo L. Ribeiro, Monique M. Gerrits, Yune H.B. Benvengo, Marco Berning, Anita P.O. Godoy, Ernst J. Kuipers, Sergio Mendonça, Arnoud H.M. Van Vliet, José Pedrazzoli, Johannes G. Kusters^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

Tetracycline is one of four antibiotics commonly used for the treatment of Helicobacter pylori infection, but its effectiveness is decreasing as the incidence of tetracycline resistance is increasing. In five Brazilian tetracycline-resistant (Tet^R) H. pylori isolates, high-level tetracycline resistance is mediated by the triple-base-pair substitution AGA_926-928→TTC in both 16S rRNA genes, as was previously observed in two independent high-level Tet^R H. pylori strains. A polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) assay was developed for the detection of the AGA_926-928→TTC substitution, and confirmed the presence of the aforementioned triple-base-pair substitution in all five Brazilian Tet^R isolates. This PCR-RFLP-based approach distinguishes the high-level Tet^R isolates from low-level Tet^R and Tet^S H. pylori strains and thus allows the direct detection of Tet^R H. pylori isolates.

Original language	English
Pages (from-to)	57-61
Number of pages	5
Journal	FEMS Immunology and Medical Microbiology
Volume	40
Issue number	1
DOIs	https://doi.org/10.1016/S0928-8244(03)00277-3
Publication status	Published - Jan 15 2004
Externally published	Yes

ASJC Scopus Subject Areas

Immunology and Allergy
Microbiology
Immunology
Microbiology (medical)
Infectious Diseases

Keywords

Antibiotic resistance
Gastric disease
Helicobacter pylori
Peptic ulcer
Polymerase chain reaction-restriction fragment length polymorphism
Tetracycline

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0928-8244(03)00277-3

Cite this

Ribeiro, M. L., Gerrits, M. M., Benvengo, Y. H. B., Berning, M., Godoy, A. P. O., Kuipers, E. J., Mendonça, S., Van Vliet, A. H. M., Pedrazzoli, J., & Kusters, J. G. (2004). Detection of high-level tetracycline resistance in clinical isolates of Helicobacter pylori using PCR-RFLP. FEMS Immunology and Medical Microbiology, 40(1), 57-61. https://doi.org/10.1016/S0928-8244(03)00277-3

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abstract = "Tetracycline is one of four antibiotics commonly used for the treatment of Helicobacter pylori infection, but its effectiveness is decreasing as the incidence of tetracycline resistance is increasing. In five Brazilian tetracycline-resistant (TetR) H. pylori isolates, high-level tetracycline resistance is mediated by the triple-base-pair substitution AGA926-928→TTC in both 16S rRNA genes, as was previously observed in two independent high-level TetR H. pylori strains. A polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) assay was developed for the detection of the AGA926-928→TTC substitution, and confirmed the presence of the aforementioned triple-base-pair substitution in all five Brazilian TetR isolates. This PCR-RFLP-based approach distinguishes the high-level TetR isolates from low-level TetR and TetS H. pylori strains and thus allows the direct detection of TetR H. pylori isolates.",

keywords = "Antibiotic resistance, Gastric disease, Helicobacter pylori, Peptic ulcer, Polymerase chain reaction-restriction fragment length polymorphism, Tetracycline",

author = "Ribeiro, {Marcelo L.} and Gerrits, {Monique M.} and Benvengo, {Yune H.B.} and Marco Berning and Godoy, {Anita P.O.} and Kuipers, {Ernst J.} and Sergio Mendon{\c c}a and {Van Vliet}, {Arnoud H.M.} and Jos{\'e} Pedrazzoli and Kusters, {Johannes G.}",

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T1 - Detection of high-level tetracycline resistance in clinical isolates of Helicobacter pylori using PCR-RFLP

AU - Ribeiro, Marcelo L.

AU - Gerrits, Monique M.

AU - Benvengo, Yune H.B.

AU - Berning, Marco

AU - Godoy, Anita P.O.

AU - Kuipers, Ernst J.

AU - Mendonça, Sergio

AU - Van Vliet, Arnoud H.M.

AU - Pedrazzoli, José

AU - Kusters, Johannes G.

PY - 2004/1/15

Y1 - 2004/1/15

N2 - Tetracycline is one of four antibiotics commonly used for the treatment of Helicobacter pylori infection, but its effectiveness is decreasing as the incidence of tetracycline resistance is increasing. In five Brazilian tetracycline-resistant (TetR) H. pylori isolates, high-level tetracycline resistance is mediated by the triple-base-pair substitution AGA926-928→TTC in both 16S rRNA genes, as was previously observed in two independent high-level TetR H. pylori strains. A polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) assay was developed for the detection of the AGA926-928→TTC substitution, and confirmed the presence of the aforementioned triple-base-pair substitution in all five Brazilian TetR isolates. This PCR-RFLP-based approach distinguishes the high-level TetR isolates from low-level TetR and TetS H. pylori strains and thus allows the direct detection of TetR H. pylori isolates.

AB - Tetracycline is one of four antibiotics commonly used for the treatment of Helicobacter pylori infection, but its effectiveness is decreasing as the incidence of tetracycline resistance is increasing. In five Brazilian tetracycline-resistant (TetR) H. pylori isolates, high-level tetracycline resistance is mediated by the triple-base-pair substitution AGA926-928→TTC in both 16S rRNA genes, as was previously observed in two independent high-level TetR H. pylori strains. A polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) assay was developed for the detection of the AGA926-928→TTC substitution, and confirmed the presence of the aforementioned triple-base-pair substitution in all five Brazilian TetR isolates. This PCR-RFLP-based approach distinguishes the high-level TetR isolates from low-level TetR and TetS H. pylori strains and thus allows the direct detection of TetR H. pylori isolates.

KW - Antibiotic resistance

KW - Gastric disease

KW - Helicobacter pylori

KW - Peptic ulcer

KW - Polymerase chain reaction-restriction fragment length polymorphism

KW - Tetracycline

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Detection of high-level tetracycline resistance in clinical isolates of Helicobacter pylori using PCR-RFLP

Abstract

ASJC Scopus Subject Areas

Keywords

UN SDGs

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