Detection of Salmonella spp. and Listeria monocytogenes in suspended organic waste by nucleic acid extraction and PCR

Carola Burtscher; Papa A. Fall; Peter A. Wilderer; Stefan Wuertz

doi:10.1128/aem.65.5.2235-2237.1999

Detection of Salmonella spp. and Listeria monocytogenes in suspended organic waste by nucleic acid extraction and PCR

Carola Burtscher, Papa A. Fall, Peter A. Wilderer, Stefan Wuertz^*

^*Corresponding author for this work

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

A nucleic acid-based method for the detection of the bacterial pathogens Salmonella spp. and Listeria monocytogenes in biological waste was developed. The detection limits were less than 10 cells per ml of biological waste. The method does not include a phenol extraction step and can be easily performed in 1 to 2 days.

Original language	English
Pages (from-to)	2235-2237
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	65
Issue number	5
DOIs	https://doi.org/10.1128/aem.65.5.2235-2237.1999
Publication status	Published - May 1999
Externally published	Yes

ASJC Scopus Subject Areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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10.1128/aem.65.5.2235-2237.1999

Cite this

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title = "Detection of Salmonella spp. and Listeria monocytogenes in suspended organic waste by nucleic acid extraction and PCR",

abstract = "A nucleic acid-based method for the detection of the bacterial pathogens Salmonella spp. and Listeria monocytogenes in biological waste was developed. The detection limits were less than 10 cells per ml of biological waste. The method does not include a phenol extraction step and can be easily performed in 1 to 2 days.",

author = "Carola Burtscher and Fall, {Papa A.} and Wilderer, {Peter A.} and Stefan Wuertz",

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AU - Wilderer, Peter A.

AU - Wuertz, Stefan

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AB - A nucleic acid-based method for the detection of the bacterial pathogens Salmonella spp. and Listeria monocytogenes in biological waste was developed. The detection limits were less than 10 cells per ml of biological waste. The method does not include a phenol extraction step and can be easily performed in 1 to 2 days.

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Detection of Salmonella spp. and Listeria monocytogenes in suspended organic waste by nucleic acid extraction and PCR

Abstract

ASJC Scopus Subject Areas

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