Development and translation of a paper-based top readout vertical flow assay for SARS-CoV-2 surveillance

Huan Jia; Eric A. Miller; Chia Ching Chan; Say Yong Ng; Mookkan Prabakaran; Meng Tao; Ian Shen Yi Cheong; Sing Mei Lim; Ming Wei Chen; Xiaohong Gao; Abirami R.; Megan E. McBee; Peter R. Preiser; Hadley D. Sikes; Patthara Kongsuphol

doi:10.1039/d2lc00073c

Development and translation of a paper-based top readout vertical flow assay for SARS-CoV-2 surveillance

Huan Jia^*, Eric A. Miller, Chia Ching Chan, Say Yong Ng, Mookkan Prabakaran, Meng Tao, Ian Shen Yi Cheong, Sing Mei Lim, Ming Wei Chen, Xiaohong Gao, Abirami R., Megan E. McBee, Peter R. Preiser, Hadley D. Sikes, Patthara Kongsuphol^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Surveillance of SARS-CoV-2 infection is critical for controlling the current pandemic. Antigen rapid tests (ARTs) provide a means for surveillance. Available lateral flow assay format ARTs rely heavily on nitrocellulose paper, raising challenges in supply shortage. Vertical flow assay (VFA) with cellulose paper as test material attracts much attention as a complementary test approach. However, current reported VFAs are facing challenges in reading the test signal from the bottom face of the test cassette, complicating the test workflow and hindering translation into rapid test application. Here, we address this gap with an enhanced VFA against SARS-CoV-2 N protein that adapts a cellulose pull-down test format allowing (1) one-step sample application at the top of the test cassette and (2) readout of the test signal from the top. We also demonstrate the feasibility of translating the enhanced VFA into a point-of-care application that can help in SARS-CoV-2 surveillance.

Original language	English
Pages (from-to)	1321-1332
Number of pages	12
Journal	Lab on a Chip
Volume	22
Issue number	7
DOIs	https://doi.org/10.1039/d2lc00073c
Publication status	Published - Feb 15 2022
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2022 The Royal Society of Chemistry.

ASJC Scopus Subject Areas

Bioengineering
Biochemistry
General Chemistry
Biomedical Engineering

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10.1039/d2lc00073c

Cite this

Jia, H., Miller, E. A., Chan, C. C., Ng, S. Y., Prabakaran, M., Tao, M., Cheong, I. S. Y., Lim, S. M., Chen, M. W., Gao, X., R., A., McBee, M. E., Preiser, P. R., Sikes, H. D., & Kongsuphol, P. (2022). Development and translation of a paper-based top readout vertical flow assay for SARS-CoV-2 surveillance. Lab on a Chip, 22(7), 1321-1332. https://doi.org/10.1039/d2lc00073c

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abstract = "Surveillance of SARS-CoV-2 infection is critical for controlling the current pandemic. Antigen rapid tests (ARTs) provide a means for surveillance. Available lateral flow assay format ARTs rely heavily on nitrocellulose paper, raising challenges in supply shortage. Vertical flow assay (VFA) with cellulose paper as test material attracts much attention as a complementary test approach. However, current reported VFAs are facing challenges in reading the test signal from the bottom face of the test cassette, complicating the test workflow and hindering translation into rapid test application. Here, we address this gap with an enhanced VFA against SARS-CoV-2 N protein that adapts a cellulose pull-down test format allowing (1) one-step sample application at the top of the test cassette and (2) readout of the test signal from the top. We also demonstrate the feasibility of translating the enhanced VFA into a point-of-care application that can help in SARS-CoV-2 surveillance.",

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Development and translation of a paper-based top readout vertical flow assay for SARS-CoV-2 surveillance

Abstract

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