Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry

Gerald Larrouy-Maumus; Abigail Clements; Alain Filloux; Ronan R. McCarthy; Serge Mostowy

doi:10.1016/j.mimet.2015.12.004

Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry

Gerald Larrouy-Maumus^*, Abigail Clements, Alain Filloux, Ronan R. McCarthy, Serge Mostowy

^*Corresponding author for this work

Imperial College London

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the matrix combined with MALDI-TOF mass spectrometry.

Original language	English
Pages (from-to)	68-71
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	120
DOIs	https://doi.org/10.1016/j.mimet.2015.12.004
Publication status	Published - Jan 1 2016
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015.

ASJC Scopus Subject Areas

Microbiology
Molecular Biology
Microbiology (medical)

Keywords

Endotoxins
Gram-negative bacteria
Mass spectrometry

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10.1016/j.mimet.2015.12.004

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title = "Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry",

abstract = "The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the matrix combined with MALDI-TOF mass spectrometry.",

keywords = "Endotoxins, Gram-negative bacteria, Mass spectrometry",

author = "Gerald Larrouy-Maumus and Abigail Clements and Alain Filloux and McCarthy, {Ronan R.} and Serge Mostowy",

note = "Publisher Copyright: {\textcopyright} 2015.",

year = "2016",

month = jan,

day = "1",

doi = "10.1016/j.mimet.2015.12.004",

language = "English",

volume = "120",

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T1 - Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry

AU - Larrouy-Maumus, Gerald

AU - Clements, Abigail

AU - Filloux, Alain

AU - McCarthy, Ronan R.

AU - Mostowy, Serge

PY - 2016/1/1

Y1 - 2016/1/1

N2 - The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the matrix combined with MALDI-TOF mass spectrometry.

AB - The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the matrix combined with MALDI-TOF mass spectrometry.

KW - Endotoxins

KW - Gram-negative bacteria

KW - Mass spectrometry

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UR - http://www.scopus.com/inward/citedby.url?scp=84949024605&partnerID=8YFLogxK

U2 - 10.1016/j.mimet.2015.12.004

DO - 10.1016/j.mimet.2015.12.004

M3 - Article

C2 - 26656001

AN - SCOPUS:84949024605

SN - 0167-7012

VL - 120

SP - 68

EP - 71

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

ER -

Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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