Discrimination of viable and dead fecal Bacteroidales bacteria by quantitative PCR with Propidium monoazide

Sungwoo Bae; Stefan Wuertz

doi:10.1128/AEM.01333-08

Discrimination of viable and dead fecal Bacteroidales bacteria by quantitative PCR with Propidium monoazide

Sungwoo Bae, Stefan Wuertz^*

^*Corresponding author for this work

University of California at Davis

Research output: Contribution to journal › Article › peer-review

182 Citations (Scopus)

Abstract

Propidium monoazide (PMA) was optimized to discriminate between viable and dead Bacteroidesfragilis cells and extracellular DNA at different concentrations of solids using quantitative PCR. Conditions of 100 μM PMA and a 10-min light exposure also excluded DNA from heat-treated cells of nonculturable Bacteroidales in human feces and wastewater influent and effluent.

Original language	English
Pages (from-to)	2940-2944
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	75
Issue number	9
DOIs	https://doi.org/10.1128/AEM.01333-08
Publication status	Published - May 2009
Externally published	Yes

ASJC Scopus Subject Areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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10.1128/AEM.01333-08

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abstract = "Propidium monoazide (PMA) was optimized to discriminate between viable and dead Bacteroidesfragilis cells and extracellular DNA at different concentrations of solids using quantitative PCR. Conditions of 100 μM PMA and a 10-min light exposure also excluded DNA from heat-treated cells of nonculturable Bacteroidales in human feces and wastewater influent and effluent.",

author = "Sungwoo Bae and Stefan Wuertz",

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AB - Propidium monoazide (PMA) was optimized to discriminate between viable and dead Bacteroidesfragilis cells and extracellular DNA at different concentrations of solids using quantitative PCR. Conditions of 100 μM PMA and a 10-min light exposure also excluded DNA from heat-treated cells of nonculturable Bacteroidales in human feces and wastewater influent and effluent.

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Discrimination of viable and dead fecal Bacteroidales bacteria by quantitative PCR with Propidium monoazide

Abstract

ASJC Scopus Subject Areas

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