DNA-derived nanostructures selectively capture gram-positive bacteria

Chan Jin Kim, Zhangyong Si, Sheethal Reghu, Zhong Guo, Kaixi Zhang, Jianghua Li, Mary B. Chan-Park*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

We report the first demonstration of the efficient bacteria targeting properties of DNA-based polymeric micelles with high-density DNA corona. Nanoscale polymer micelles derived from DNA-b-polystyrene (DNA-b-PS) efficiently selected most tested Gram-positive strains over Gram-negative strains; single-strand DNAs were 20-fold less selective. We demonstrate that these targeting properties were derived from the interaction between densely packed DNA strands of the micelle corona and the peptidoglycan layers of Gram-positive bacteria. DNA-b-PS micelles incorporating magnetic nanoparticles (MNPs) can efficiently capture and concentrate Gram-positive bacteria suggesting the simple applications of these DNA block copolymer micelles for concentrating bacteria. Adenine (A), thymine (T), cytosine (C), and guanine (G)-rich nanostructures were fabricated, respectively, for investigating the effect of sequence on Gram-selective bacteria targeting. T-rich micelles showed the most efficient targeting properties. The targeting properties of these DNA nanostructures toward Gram-positive bacteria may have applications as a targeted therapeutic delivery system. Graphical abstract: [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)1438-1450
Number of pages13
JournalDrug Delivery and Translational Research
Volume11
Issue number4
DOIs
Publication statusPublished - Aug 2021
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2021, Controlled Release Society.

ASJC Scopus Subject Areas

  • Pharmaceutical Science

Keywords

  • Bacteria capturing
  • Bacteria targeting
  • DNA block copolymer
  • Magnetic nanoparticles
  • Peptidoglycan

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