Abstract
We report the first demonstration of the efficient bacteria targeting properties of DNA-based polymeric micelles with high-density DNA corona. Nanoscale polymer micelles derived from DNA-b-polystyrene (DNA-b-PS) efficiently selected most tested Gram-positive strains over Gram-negative strains; single-strand DNAs were 20-fold less selective. We demonstrate that these targeting properties were derived from the interaction between densely packed DNA strands of the micelle corona and the peptidoglycan layers of Gram-positive bacteria. DNA-b-PS micelles incorporating magnetic nanoparticles (MNPs) can efficiently capture and concentrate Gram-positive bacteria suggesting the simple applications of these DNA block copolymer micelles for concentrating bacteria. Adenine (A), thymine (T), cytosine (C), and guanine (G)-rich nanostructures were fabricated, respectively, for investigating the effect of sequence on Gram-selective bacteria targeting. T-rich micelles showed the most efficient targeting properties. The targeting properties of these DNA nanostructures toward Gram-positive bacteria may have applications as a targeted therapeutic delivery system. Graphical abstract: [Figure not available: see fulltext.]
Original language | English |
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Pages (from-to) | 1438-1450 |
Number of pages | 13 |
Journal | Drug Delivery and Translational Research |
Volume | 11 |
Issue number | 4 |
DOIs | |
Publication status | Published - Aug 2021 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2021, Controlled Release Society.
ASJC Scopus Subject Areas
- Pharmaceutical Science
Keywords
- Bacteria capturing
- Bacteria targeting
- DNA block copolymer
- Magnetic nanoparticles
- Peptidoglycan