Effects of specimen refractive index on confocal imaging

C. J.R. Sheppard; P. Török

doi:10.1046/j.1365-2818.1997.d01-627.x

Effects of specimen refractive index on confocal imaging

C. J.R. Sheppard^*, P. Török

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

90 Citations (Scopus)

Abstract

The aberrations introduced when focusing within a specimen with a refractive index equal to that of water using an oil-immersion objective are investigated theoretically. The peak intensity in the confocal point spread function drops by a factor of two for focusing less than 10 μm into the specimen. The effects on scaling of dimensions in the resulting images are discussed. The image exhibits an axial stretching by a factor of about 1.12.

Original language	English
Pages (from-to)	366-374
Number of pages	9
Journal	Journal of Microscopy
Volume	185
Issue number	3
DOIs	https://doi.org/10.1046/j.1365-2818.1997.d01-627.x
Publication status	Published - 1997
Externally published	Yes

ASJC Scopus Subject Areas

Pathology and Forensic Medicine
Histology

Keywords

Aberration
Confocal microscopy
Point spread function

Access to Document

10.1046/j.1365-2818.1997.d01-627.x

Cite this

@article{beda707cb7c441f2a7d46f3180a604b5,

title = "Effects of specimen refractive index on confocal imaging",

abstract = "The aberrations introduced when focusing within a specimen with a refractive index equal to that of water using an oil-immersion objective are investigated theoretically. The peak intensity in the confocal point spread function drops by a factor of two for focusing less than 10 μm into the specimen. The effects on scaling of dimensions in the resulting images are discussed. The image exhibits an axial stretching by a factor of about 1.12.",

keywords = "Aberration, Confocal microscopy, Point spread function",

author = "Sheppard, \{C. J.R.\} and P. T{\"o}r{\"o}k",

year = "1997",

doi = "10.1046/j.1365-2818.1997.d01-627.x",

language = "English",

volume = "185",

pages = "366--374",

journal = "Journal of Microscopy",

issn = "0022-2720",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - Effects of specimen refractive index on confocal imaging

AU - Sheppard, C. J.R.

AU - Török, P.

PY - 1997

Y1 - 1997

N2 - The aberrations introduced when focusing within a specimen with a refractive index equal to that of water using an oil-immersion objective are investigated theoretically. The peak intensity in the confocal point spread function drops by a factor of two for focusing less than 10 μm into the specimen. The effects on scaling of dimensions in the resulting images are discussed. The image exhibits an axial stretching by a factor of about 1.12.

AB - The aberrations introduced when focusing within a specimen with a refractive index equal to that of water using an oil-immersion objective are investigated theoretically. The peak intensity in the confocal point spread function drops by a factor of two for focusing less than 10 μm into the specimen. The effects on scaling of dimensions in the resulting images are discussed. The image exhibits an axial stretching by a factor of about 1.12.

KW - Aberration

KW - Confocal microscopy

KW - Point spread function

UR - http://www.scopus.com/inward/record.url?scp=0030895212&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030895212&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2818.1997.d01-627.x

DO - 10.1046/j.1365-2818.1997.d01-627.x

M3 - Article

AN - SCOPUS:0030895212

SN - 0022-2720

VL - 185

SP - 366

EP - 374

JO - Journal of Microscopy

JF - Journal of Microscopy

IS - 3

ER -

Effects of specimen refractive index on confocal imaging

Abstract

ASJC Scopus Subject Areas

Keywords

Access to Document

Other files and links

Fingerprint

Cite this