Electrochemical Quantification of Escherichia coli with DNA Nanostructure

Marcella Giovanni; Magdiel Inggrid Setyawati; Chor Yong Tay; Hang Qian; Win Sen Kuan; David Tai Leong

doi:10.1002/adfm.201500940

Electrochemical Quantification of Escherichia coli with DNA Nanostructure

Marcella Giovanni, Magdiel Inggrid Setyawati, Chor Yong Tay, Hang Qian, Win Sen Kuan^*, David Tai Leong

^*Corresponding author for this work

National University of Singapore

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Abstract

Escherichia coli is an indicator of fecal contamination in recreational water and some strains are responsible for serious food poisoning in humans. Therefore, having a rapid, sensitive, selective, and simple E. coli detection is important. In this study, DNA nanopyramids anchor is utilized for immunological detection of E. coli lipopolysaccharides, lysate, and whole bacteria. By measuring the E. coli amount electrochemically, detection limits of 0.20 ng mL^-1E. coli lipopolysaccharides and 1.20 CFU mL^-1 equivalent of lysed E. coli bacteria are obtained. Our method confers selectivity to detect E. coli in the presence of various other macromolecules. Therefore, our system can be developed to test the E. coli content in real water samples.

Original language	English
Pages (from-to)	3840-3846
Number of pages	7
Journal	Advanced Functional Materials
Volume	25
Issue number	25
DOIs	https://doi.org/10.1002/adfm.201500940
Publication status	Published - Jul 1 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

ASJC Scopus Subject Areas

Electronic, Optical and Magnetic Materials
General Chemistry
Biomaterials
General Materials Science
Condensed Matter Physics
Electrochemistry

Keywords

biosensor
DNA nanostructures
Escherichia coli

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10.1002/adfm.201500940

Cite this

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title = "Electrochemical Quantification of Escherichia coli with DNA Nanostructure",

abstract = "Escherichia coli is an indicator of fecal contamination in recreational water and some strains are responsible for serious food poisoning in humans. Therefore, having a rapid, sensitive, selective, and simple E. coli detection is important. In this study, DNA nanopyramids anchor is utilized for immunological detection of E. coli lipopolysaccharides, lysate, and whole bacteria. By measuring the E. coli amount electrochemically, detection limits of 0.20 ng mL-1E. coli lipopolysaccharides and 1.20 CFU mL-1 equivalent of lysed E. coli bacteria are obtained. Our method confers selectivity to detect E. coli in the presence of various other macromolecules. Therefore, our system can be developed to test the E. coli content in real water samples.",

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doi = "10.1002/adfm.201500940",

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T1 - Electrochemical Quantification of Escherichia coli with DNA Nanostructure

AU - Giovanni, Marcella

AU - Setyawati, Magdiel Inggrid

AU - Tay, Chor Yong

AU - Qian, Hang

AU - Kuan, Win Sen

AU - Leong, David Tai

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Escherichia coli is an indicator of fecal contamination in recreational water and some strains are responsible for serious food poisoning in humans. Therefore, having a rapid, sensitive, selective, and simple E. coli detection is important. In this study, DNA nanopyramids anchor is utilized for immunological detection of E. coli lipopolysaccharides, lysate, and whole bacteria. By measuring the E. coli amount electrochemically, detection limits of 0.20 ng mL-1E. coli lipopolysaccharides and 1.20 CFU mL-1 equivalent of lysed E. coli bacteria are obtained. Our method confers selectivity to detect E. coli in the presence of various other macromolecules. Therefore, our system can be developed to test the E. coli content in real water samples.

AB - Escherichia coli is an indicator of fecal contamination in recreational water and some strains are responsible for serious food poisoning in humans. Therefore, having a rapid, sensitive, selective, and simple E. coli detection is important. In this study, DNA nanopyramids anchor is utilized for immunological detection of E. coli lipopolysaccharides, lysate, and whole bacteria. By measuring the E. coli amount electrochemically, detection limits of 0.20 ng mL-1E. coli lipopolysaccharides and 1.20 CFU mL-1 equivalent of lysed E. coli bacteria are obtained. Our method confers selectivity to detect E. coli in the presence of various other macromolecules. Therefore, our system can be developed to test the E. coli content in real water samples.

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KW - DNA nanostructures

KW - Escherichia coli

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Electrochemical Quantification of Escherichia coli with DNA Nanostructure

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

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