Exploiting Caustics for Snapshot 3D Fluorescence Imaging in Wide-field Microscopy

Sanjeev Kumar; Zheng Li; Chongquan Huang; Vincent Lin; Sing Yian Chew; Rajeev J. Ram

doi:10.1364/COSI.2023.CM3B.6

Exploiting Caustics for Snapshot 3D Fluorescence Imaging in Wide-field Microscopy

Sanjeev Kumar, Zheng Li, Chongquan Huang, Vincent Lin, Sing Yian Chew, Rajeev J. Ram^*

^*Corresponding author for this work

Research output: Contribution to conference › Paper › peer-review

Abstract

We present a snapshot 3D fluorescence microscopy technique that extracts depth from defocus by exploiting caustics generated when spherical aberration is intentionally introduced in a high-numerical aperture microscope. The snapshot acquisition reduces the imaging time and the risk of phototoxicity associated with scanning based methods.

Original language	English
DOIs	https://doi.org/10.1364/COSI.2023.CM3B.6
Publication status	Published - 2023
Externally published	Yes
Event	2023 Computational Optical Sensing and Imaging, COSI 2023 in Optica Imaging Congress - Part of Imaging and Applied Optics Congress 2023 - Boston, United States Duration: Aug 14 2023 → Aug 17 2023

Conference

Conference	2023 Computational Optical Sensing and Imaging, COSI 2023 in Optica Imaging Congress - Part of Imaging and Applied Optics Congress 2023
Country/Territory	United States
City	Boston
Period	8/14/23 → 8/17/23

Bibliographical note

Publisher Copyright:
© 2023 The Author(s).

ASJC Scopus Subject Areas

General Computer Science
Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Space and Planetary Science
Control and Systems Engineering
Electrical and Electronic Engineering
Instrumentation

Access to Document

10.1364/COSI.2023.CM3B.6

Cite this

Kumar, S., Li, Z., Huang, C., Lin, V., Chew, S. Y., & Ram, R. J. (2023). Exploiting Caustics for Snapshot 3D Fluorescence Imaging in Wide-field Microscopy. Paper presented at 2023 Computational Optical Sensing and Imaging, COSI 2023 in Optica Imaging Congress - Part of Imaging and Applied Optics Congress 2023, Boston, United States. https://doi.org/10.1364/COSI.2023.CM3B.6

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title = "Exploiting Caustics for Snapshot 3D Fluorescence Imaging in Wide-field Microscopy",

abstract = "We present a snapshot 3D fluorescence microscopy technique that extracts depth from defocus by exploiting caustics generated when spherical aberration is intentionally introduced in a high-numerical aperture microscope. The snapshot acquisition reduces the imaging time and the risk of phototoxicity associated with scanning based methods.",

author = "Sanjeev Kumar and Zheng Li and Chongquan Huang and Vincent Lin and Chew, {Sing Yian} and Ram, {Rajeev J.}",

note = "Publisher Copyright: {\textcopyright} 2023 The Author(s).; 2023 Computational Optical Sensing and Imaging, COSI 2023 in Optica Imaging Congress - Part of Imaging and Applied Optics Congress 2023 ; Conference date: 14-08-2023 Through 17-08-2023",

year = "2023",

doi = "10.1364/COSI.2023.CM3B.6",

language = "English",

}

Kumar, S, Li, Z, Huang, C, Lin, V, Chew, SY & Ram, RJ 2023, 'Exploiting Caustics for Snapshot 3D Fluorescence Imaging in Wide-field Microscopy', Paper presented at 2023 Computational Optical Sensing and Imaging, COSI 2023 in Optica Imaging Congress - Part of Imaging and Applied Optics Congress 2023, Boston, United States, 8/14/23 - 8/17/23. https://doi.org/10.1364/COSI.2023.CM3B.6

Exploiting Caustics for Snapshot 3D Fluorescence Imaging in Wide-field Microscopy. / Kumar, Sanjeev; Li, Zheng; Huang, Chongquan et al.
2023. Paper presented at 2023 Computational Optical Sensing and Imaging, COSI 2023 in Optica Imaging Congress - Part of Imaging and Applied Optics Congress 2023, Boston, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Exploiting Caustics for Snapshot 3D Fluorescence Imaging in Wide-field Microscopy

AU - Kumar, Sanjeev

AU - Li, Zheng

AU - Huang, Chongquan

AU - Lin, Vincent

AU - Chew, Sing Yian

AU - Ram, Rajeev J.

PY - 2023

Y1 - 2023

N2 - We present a snapshot 3D fluorescence microscopy technique that extracts depth from defocus by exploiting caustics generated when spherical aberration is intentionally introduced in a high-numerical aperture microscope. The snapshot acquisition reduces the imaging time and the risk of phototoxicity associated with scanning based methods.

AB - We present a snapshot 3D fluorescence microscopy technique that extracts depth from defocus by exploiting caustics generated when spherical aberration is intentionally introduced in a high-numerical aperture microscope. The snapshot acquisition reduces the imaging time and the risk of phototoxicity associated with scanning based methods.

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DO - 10.1364/COSI.2023.CM3B.6

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T2 - 2023 Computational Optical Sensing and Imaging, COSI 2023 in Optica Imaging Congress - Part of Imaging and Applied Optics Congress 2023

Y2 - 14 August 2023 through 17 August 2023

ER -

Exploiting Caustics for Snapshot 3D Fluorescence Imaging in Wide-field Microscopy

Abstract

Conference

Bibliographical note

ASJC Scopus Subject Areas

Access to Document

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