TY - JOUR
T1 - Expression, purification and preliminary crystallographic analysis of Pseudomonas aeruginosa RocR protein
AU - Kotaka, Masayo
AU - Dutta, Sujit
AU - Lee, Hooi Chen
AU - Lim, Mitchell J.M.
AU - Wong, Yeehwa
AU - Rao, Feng
AU - Mitchell, Edward P.
AU - Liang, Zhao Xun
AU - Lescar, Julien
PY - 2009
Y1 - 2009
N2 - Pseudomonas aeruginosa RocR, an EAL-domain protein which regulates the expression of virulence genes and biofilm formation, has been cloned and expressed in Escherichia coli and purified. Here, the crystallization and preliminary diffraction analysis of RocR are reported. The X-ray diffraction data were processed to a resolution of 2.50 Å. The crystals belonged to space group P6122 or P6522, with unit-cell parameters a = 118.8, b = 118.8, c = 495.1 Å, = Β = 90, = 120°.
AB - Pseudomonas aeruginosa RocR, an EAL-domain protein which regulates the expression of virulence genes and biofilm formation, has been cloned and expressed in Escherichia coli and purified. Here, the crystallization and preliminary diffraction analysis of RocR are reported. The X-ray diffraction data were processed to a resolution of 2.50 Å. The crystals belonged to space group P6122 or P6522, with unit-cell parameters a = 118.8, b = 118.8, c = 495.1 Å, = Β = 90, = 120°.
KW - EAL-domain proteins
KW - Pseudomonas aeruginosa
KW - RocR
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U2 - 10.1107/S1744309109034526
DO - 10.1107/S1744309109034526
M3 - Article
C2 - 19851016
AN - SCOPUS:70350331582
SN - 1744-3091
VL - 65
SP - 1035
EP - 1038
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 10
ER -