Fluoro-photoacoustic polymeric renal reporter for real-time dual imaging of acute kidney injury

Penghui Cheng; Kanyi Pu

doi:10.1016/bs.mie.2021.06.020

Fluoro-photoacoustic polymeric renal reporter for real-time dual imaging of acute kidney injury

Penghui Cheng, Kanyi Pu^*

^*Corresponding author for this work

Nanyang Technological University

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Citations (Scopus)

Abstract

In this chapter, we introduced a diagnostic approach for acute kidney injury (AKI) via photoacoustic imaging. We provided detailed synthetic procedures of a biomarker-activatable photoacoustic agent (FPRR) with high renal clearance efficiency. We also provided protocols for in vitro characterization, live-cell imaging, and in vivo imaging in a drug-induced AKI mice model. Compared to near-infrared fluorescence imaging, photoacoustic imaging is more sensitive with higher signal-to-background ratio. As such, our approach serves as a general guideline in the development of photoacoustic agents for diagnosis of urological diseases. With this tool in hand, researchers in the field of optical imaging may be inspired to develop other photoacoustic agents for early stage disease diagnosis.

Original language	English
Title of host publication	Photoacoustic Probes for In Vivo Imaging
Editors	Jefferson Chan
Publisher	Academic Press Inc.
Pages	271-300
Number of pages	30
ISBN (Print)	9780323855303
DOIs	https://doi.org/10.1016/bs.mie.2021.06.020
Publication status	Published - Jan 2021
Externally published	Yes

Publication series

Name	Methods in Enzymology
Volume	657
ISSN (Print)	0076-6879
ISSN (Electronic)	1557-7988

Bibliographical note

Publisher Copyright:
© 2021 Elsevier Inc.

ASJC Scopus Subject Areas

Biochemistry
Molecular Biology

Keywords

Acute kidney injury
Disease diagnosis
Dual channel imaging
Real-time photoacoustic imaging
Renal clearance

Access to Document

10.1016/bs.mie.2021.06.020

Cite this

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title = "Fluoro-photoacoustic polymeric renal reporter for real-time dual imaging of acute kidney injury",

abstract = "In this chapter, we introduced a diagnostic approach for acute kidney injury (AKI) via photoacoustic imaging. We provided detailed synthetic procedures of a biomarker-activatable photoacoustic agent (FPRR) with high renal clearance efficiency. We also provided protocols for in vitro characterization, live-cell imaging, and in vivo imaging in a drug-induced AKI mice model. Compared to near-infrared fluorescence imaging, photoacoustic imaging is more sensitive with higher signal-to-background ratio. As such, our approach serves as a general guideline in the development of photoacoustic agents for diagnosis of urological diseases. With this tool in hand, researchers in the field of optical imaging may be inspired to develop other photoacoustic agents for early stage disease diagnosis.",

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author = "Penghui Cheng and Kanyi Pu",

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AU - Pu, Kanyi

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AB - In this chapter, we introduced a diagnostic approach for acute kidney injury (AKI) via photoacoustic imaging. We provided detailed synthetic procedures of a biomarker-activatable photoacoustic agent (FPRR) with high renal clearance efficiency. We also provided protocols for in vitro characterization, live-cell imaging, and in vivo imaging in a drug-induced AKI mice model. Compared to near-infrared fluorescence imaging, photoacoustic imaging is more sensitive with higher signal-to-background ratio. As such, our approach serves as a general guideline in the development of photoacoustic agents for diagnosis of urological diseases. With this tool in hand, researchers in the field of optical imaging may be inspired to develop other photoacoustic agents for early stage disease diagnosis.

KW - Acute kidney injury

KW - Disease diagnosis

KW - Dual channel imaging

KW - Real-time photoacoustic imaging

KW - Renal clearance

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M3 - Chapter

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Fluoro-photoacoustic polymeric renal reporter for real-time dual imaging of acute kidney injury

Abstract

Publication series

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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