Free nitrous acid inhibition on nitrous oxide reduction by a denitrifying-enhanced biological phosphorus removal sludge

Yan Zhou; Maite Pijuan; Raymond J. Zeng; Zhiguo Yuan

doi:10.1021/es800650j

Free nitrous acid inhibition on nitrous oxide reduction by a denitrifying-enhanced biological phosphorus removal sludge

Yan Zhou, Maite Pijuan, Raymond J. Zeng, Zhiguo Yuan^*

^*Corresponding author for this work

University of Queensland

Research output: Contribution to journal › Article › peer-review

262 Citations (Scopus)

Abstract

Nitrite has generally been recognized as an inhibitor of N₂O reduction during denitrification. This inhibitory effect is investigated under various pH conditions using a denitrifying-enhanced biological phosphorus removal (EBPR) sludge. The degree of inhibition was observed to correlate much more strongly with the free nitrous acid (FNA) concentration than with the nitrite concentration, suggesting that FNA, rather than nitrite, is likely the true inhibitor on N₂O reduction. Fifty percent inhibition was observed at an FNA concentration of 0.0007-0.001 mg HNO₂-N/L (equivalent to approximately 3-4 mg NO₂^--N/L at pH 7), while complete inhibition occurred when the FNA concentration was greater than 0.004 mg HNO₂-N/L The results also suggest that the inhibition on N₂O reduction was not due to the electron competition between N ₂O and NO₂^- reductases. The inhibition was found to be reversible, with the rate of recovery independent of the duration of the inhibition, but dependent on the concentration of FNA the biomass was exposed to during the inhibition period. A higher FNA concentration caused slower recovery.

Original language	English
Pages (from-to)	8260-8265
Number of pages	6
Journal	Environmental Science and Technology
Volume	42
Issue number	22
DOIs	https://doi.org/10.1021/es800650j
Publication status	Published - Nov 15 2008
Externally published	Yes

ASJC Scopus Subject Areas

General Chemistry
Environmental Chemistry

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title = "Free nitrous acid inhibition on nitrous oxide reduction by a denitrifying-enhanced biological phosphorus removal sludge",

abstract = "Nitrite has generally been recognized as an inhibitor of N2O reduction during denitrification. This inhibitory effect is investigated under various pH conditions using a denitrifying-enhanced biological phosphorus removal (EBPR) sludge. The degree of inhibition was observed to correlate much more strongly with the free nitrous acid (FNA) concentration than with the nitrite concentration, suggesting that FNA, rather than nitrite, is likely the true inhibitor on N2O reduction. Fifty percent inhibition was observed at an FNA concentration of 0.0007-0.001 mg HNO2-N/L (equivalent to approximately 3-4 mg NO2--N/L at pH 7), while complete inhibition occurred when the FNA concentration was greater than 0.004 mg HNO2-N/L The results also suggest that the inhibition on N2O reduction was not due to the electron competition between N 2O and NO2- reductases. The inhibition was found to be reversible, with the rate of recovery independent of the duration of the inhibition, but dependent on the concentration of FNA the biomass was exposed to during the inhibition period. A higher FNA concentration caused slower recovery.",

author = "Yan Zhou and Maite Pijuan and Zeng, {Raymond J.} and Zhiguo Yuan",

year = "2008",

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T1 - Free nitrous acid inhibition on nitrous oxide reduction by a denitrifying-enhanced biological phosphorus removal sludge

AU - Zhou, Yan

AU - Pijuan, Maite

AU - Zeng, Raymond J.

AU - Yuan, Zhiguo

PY - 2008/11/15

Y1 - 2008/11/15

N2 - Nitrite has generally been recognized as an inhibitor of N2O reduction during denitrification. This inhibitory effect is investigated under various pH conditions using a denitrifying-enhanced biological phosphorus removal (EBPR) sludge. The degree of inhibition was observed to correlate much more strongly with the free nitrous acid (FNA) concentration than with the nitrite concentration, suggesting that FNA, rather than nitrite, is likely the true inhibitor on N2O reduction. Fifty percent inhibition was observed at an FNA concentration of 0.0007-0.001 mg HNO2-N/L (equivalent to approximately 3-4 mg NO2--N/L at pH 7), while complete inhibition occurred when the FNA concentration was greater than 0.004 mg HNO2-N/L The results also suggest that the inhibition on N2O reduction was not due to the electron competition between N 2O and NO2- reductases. The inhibition was found to be reversible, with the rate of recovery independent of the duration of the inhibition, but dependent on the concentration of FNA the biomass was exposed to during the inhibition period. A higher FNA concentration caused slower recovery.

AB - Nitrite has generally been recognized as an inhibitor of N2O reduction during denitrification. This inhibitory effect is investigated under various pH conditions using a denitrifying-enhanced biological phosphorus removal (EBPR) sludge. The degree of inhibition was observed to correlate much more strongly with the free nitrous acid (FNA) concentration than with the nitrite concentration, suggesting that FNA, rather than nitrite, is likely the true inhibitor on N2O reduction. Fifty percent inhibition was observed at an FNA concentration of 0.0007-0.001 mg HNO2-N/L (equivalent to approximately 3-4 mg NO2--N/L at pH 7), while complete inhibition occurred when the FNA concentration was greater than 0.004 mg HNO2-N/L The results also suggest that the inhibition on N2O reduction was not due to the electron competition between N 2O and NO2- reductases. The inhibition was found to be reversible, with the rate of recovery independent of the duration of the inhibition, but dependent on the concentration of FNA the biomass was exposed to during the inhibition period. A higher FNA concentration caused slower recovery.

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Free nitrous acid inhibition on nitrous oxide reduction by a denitrifying-enhanced biological phosphorus removal sludge

Abstract

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