Full circumferential morphological analysis of Schlemm’s canal in human eyes using megahertz swept source OCT

Xinwen Yao; Bingyao Tan; Yijie Ho; Xinyu Liu; Damon Wong; Jacqueline Chua; Tina T. Wong; Shamira Perera; Marcus Ang; René M. Werkmeister; Leopold Schmetterer

doi:10.1364/BOE.426218

Full circumferential morphological analysis of Schlemm’s canal in human eyes using megahertz swept source OCT

Xinwen Yao, Bingyao Tan, Yijie Ho, Xinyu Liu, Damon Wong, Jacqueline Chua, Tina T. Wong, Shamira Perera, Marcus Ang, René M. Werkmeister, Leopold Schmetterer^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

We performed full circumferential imaging of the Schlemm’s canal (SC) of two human eyes using a Fourier domain mode-lock laser (FDML) based 1.66-MHz SS-OCT prototype at 1060 nm. Eight volumes with overlapping margins were acquired around the limbal area with customized raster scanning patterns designed to fully cover the SC while minimizing motion artifacts. The SC was segmented from the volumes using a semi-automated active contour segmentation algorithm, whose mean dice similarity coefficient was 0.76 compared to the manual segmentation results. We also reconstructed three-dimensional (3D) renderings of the 360° SC by stitching the segmented SCs from the volumetric datasets. Quantitative metrics of the full circumferential SC were provided, including the mean and standard deviation (SD) of the cross-sectional area (CSA), the maximum CSA, the minimum and maximum SC opening width, and the number of collector channels (CC) stemming from the SC.

Original language	English
Pages (from-to)	3865-3877
Number of pages	13
Journal	Biomedical Optics Express
Volume	12
Issue number	7
DOIs	https://doi.org/10.1364/BOE.426218
Publication status	Published - Jul 1 2021
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

ASJC Scopus Subject Areas

Biotechnology
Atomic and Molecular Physics, and Optics

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title = "Full circumferential morphological analysis of Schlemm{\textquoteright}s canal in human eyes using megahertz swept source OCT",

abstract = "We performed full circumferential imaging of the Schlemm{\textquoteright}s canal (SC) of two human eyes using a Fourier domain mode-lock laser (FDML) based 1.66-MHz SS-OCT prototype at 1060 nm. Eight volumes with overlapping margins were acquired around the limbal area with customized raster scanning patterns designed to fully cover the SC while minimizing motion artifacts. The SC was segmented from the volumes using a semi-automated active contour segmentation algorithm, whose mean dice similarity coefficient was 0.76 compared to the manual segmentation results. We also reconstructed three-dimensional (3D) renderings of the 360° SC by stitching the segmented SCs from the volumetric datasets. Quantitative metrics of the full circumferential SC were provided, including the mean and standard deviation (SD) of the cross-sectional area (CSA), the maximum CSA, the minimum and maximum SC opening width, and the number of collector channels (CC) stemming from the SC.",

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doi = "10.1364/BOE.426218",

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T1 - Full circumferential morphological analysis of Schlemm’s canal in human eyes using megahertz swept source OCT

AU - Yao, Xinwen

AU - Tan, Bingyao

AU - Ho, Yijie

AU - Liu, Xinyu

AU - Wong, Damon

AU - Chua, Jacqueline

AU - Wong, Tina T.

AU - Perera, Shamira

AU - Ang, Marcus

AU - Werkmeister, René M.

AU - Schmetterer, Leopold

PY - 2021/7/1

Y1 - 2021/7/1

N2 - We performed full circumferential imaging of the Schlemm’s canal (SC) of two human eyes using a Fourier domain mode-lock laser (FDML) based 1.66-MHz SS-OCT prototype at 1060 nm. Eight volumes with overlapping margins were acquired around the limbal area with customized raster scanning patterns designed to fully cover the SC while minimizing motion artifacts. The SC was segmented from the volumes using a semi-automated active contour segmentation algorithm, whose mean dice similarity coefficient was 0.76 compared to the manual segmentation results. We also reconstructed three-dimensional (3D) renderings of the 360° SC by stitching the segmented SCs from the volumetric datasets. Quantitative metrics of the full circumferential SC were provided, including the mean and standard deviation (SD) of the cross-sectional area (CSA), the maximum CSA, the minimum and maximum SC opening width, and the number of collector channels (CC) stemming from the SC.

AB - We performed full circumferential imaging of the Schlemm’s canal (SC) of two human eyes using a Fourier domain mode-lock laser (FDML) based 1.66-MHz SS-OCT prototype at 1060 nm. Eight volumes with overlapping margins were acquired around the limbal area with customized raster scanning patterns designed to fully cover the SC while minimizing motion artifacts. The SC was segmented from the volumes using a semi-automated active contour segmentation algorithm, whose mean dice similarity coefficient was 0.76 compared to the manual segmentation results. We also reconstructed three-dimensional (3D) renderings of the 360° SC by stitching the segmented SCs from the volumetric datasets. Quantitative metrics of the full circumferential SC were provided, including the mean and standard deviation (SD) of the cross-sectional area (CSA), the maximum CSA, the minimum and maximum SC opening width, and the number of collector channels (CC) stemming from the SC.

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Full circumferential morphological analysis of Schlemm’s canal in human eyes using megahertz swept source OCT

Abstract

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