GapmeR-mediated gene silencing in motile T-cells

Mobashar Hussain Urf Turabe Fazil; Seow Theng Ong; Madhavi Latha Somaraju Chalasani; Atish Kizhakeyil; Navin Kumar Verma

doi:10.1007/978-1-4939-9036-8_9

GapmeR-mediated gene silencing in motile T-cells

Mobashar Hussain Urf Turabe Fazil^*, Seow Theng Ong, Madhavi Latha Somaraju Chalasani, Atish Kizhakeyil, Navin Kumar Verma

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

Gene silencing is an important method to study gene functions in health and diseases. While there are various techniques that are applied to knockdown specific gene(s) of interest, they have certain limitations in application to T-lymphocytes. T-cells are “hard-to-transfect” cells and are recalcitrant to transfection reagents. Here, we describe the use of novel cell-permeating antisense molecules, called “GapmeR”, to knockdown specific gene(s) in human primary T-cells.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	67-73
Number of pages	7
DOIs	https://doi.org/10.1007/978-1-4939-9036-8_9
Publication status	Published - 2019
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1930
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

Keywords

GapmeR
Gene knockdown
RNAi
T-Lymphocytes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-4939-9036-8_9

Cite this

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abstract = "Gene silencing is an important method to study gene functions in health and diseases. While there are various techniques that are applied to knockdown specific gene(s) of interest, they have certain limitations in application to T-lymphocytes. T-cells are “hard-to-transfect” cells and are recalcitrant to transfection reagents. Here, we describe the use of novel cell-permeating antisense molecules, called “GapmeR”, to knockdown specific gene(s) in human primary T-cells.",

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AU - Fazil, Mobashar Hussain Urf Turabe

AU - Ong, Seow Theng

AU - Chalasani, Madhavi Latha Somaraju

AU - Kizhakeyil, Atish

AU - Verma, Navin Kumar

PY - 2019

Y1 - 2019

N2 - Gene silencing is an important method to study gene functions in health and diseases. While there are various techniques that are applied to knockdown specific gene(s) of interest, they have certain limitations in application to T-lymphocytes. T-cells are “hard-to-transfect” cells and are recalcitrant to transfection reagents. Here, we describe the use of novel cell-permeating antisense molecules, called “GapmeR”, to knockdown specific gene(s) in human primary T-cells.

AB - Gene silencing is an important method to study gene functions in health and diseases. While there are various techniques that are applied to knockdown specific gene(s) of interest, they have certain limitations in application to T-lymphocytes. T-cells are “hard-to-transfect” cells and are recalcitrant to transfection reagents. Here, we describe the use of novel cell-permeating antisense molecules, called “GapmeR”, to knockdown specific gene(s) in human primary T-cells.

KW - GapmeR

KW - Gene knockdown

KW - RNAi

KW - T-Lymphocytes

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T3 - Methods in Molecular Biology

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

GapmeR-mediated gene silencing in motile T-cells

Abstract

Publication series

Bibliographical note

ASJC Scopus Subject Areas

Keywords

UN SDGs

Access to Document

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