Gene amplification and qRT-PCR

Cerith Jones; Alain Filloux

doi:10.1007/978-1-4939-0473-0_35

Gene amplification and qRT-PCR

Cerith Jones, Alain Filloux

President's Office

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

This chapter includes methods for the use of the polymerase chain reaction (PCR) with Pseudomonas, and several specific tips for their successful application in this organism. The first part of the chapter includes methods for purifying genomic DNA from, and amplifying genes from, Pseudomonas, in addition to methods which describe how to prepare a cell lysate from Pseudomonas species for colony PCR reactions. The chapter continues with a switch in focus from DNA to RNA, describing methods for RNA isolation from Pseudomonas, cDNA generation, and finally q-RT-PCR to investigate relative changes in gene expression.

Original language	English
Pages (from-to)	457-468
Number of pages	12
Journal	Methods in Molecular Biology
Volume	1149
DOIs	https://doi.org/10.1007/978-1-4939-0473-0_35
Publication status	Published - 2014

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2014.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

Keywords

CDNA
Cloning
Colony PCR
Gene amplification
PCR
QRT-PCR
RNA
RT-PCR

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10.1007/978-1-4939-0473-0_35

Cite this

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title = "Gene amplification and qRT-PCR",

abstract = "This chapter includes methods for the use of the polymerase chain reaction (PCR) with Pseudomonas, and several specific tips for their successful application in this organism. The first part of the chapter includes methods for purifying genomic DNA from, and amplifying genes from, Pseudomonas, in addition to methods which describe how to prepare a cell lysate from Pseudomonas species for colony PCR reactions. The chapter continues with a switch in focus from DNA to RNA, describing methods for RNA isolation from Pseudomonas, cDNA generation, and finally q-RT-PCR to investigate relative changes in gene expression.",

keywords = "CDNA, Cloning, Colony PCR, Gene amplification, PCR, QRT-PCR, RNA, RT-PCR",

author = "Cerith Jones and Alain Filloux",

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AU - Filloux, Alain

N1 - Publisher Copyright: © Springer Science+Business Media New York 2014.

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N2 - This chapter includes methods for the use of the polymerase chain reaction (PCR) with Pseudomonas, and several specific tips for their successful application in this organism. The first part of the chapter includes methods for purifying genomic DNA from, and amplifying genes from, Pseudomonas, in addition to methods which describe how to prepare a cell lysate from Pseudomonas species for colony PCR reactions. The chapter continues with a switch in focus from DNA to RNA, describing methods for RNA isolation from Pseudomonas, cDNA generation, and finally q-RT-PCR to investigate relative changes in gene expression.

AB - This chapter includes methods for the use of the polymerase chain reaction (PCR) with Pseudomonas, and several specific tips for their successful application in this organism. The first part of the chapter includes methods for purifying genomic DNA from, and amplifying genes from, Pseudomonas, in addition to methods which describe how to prepare a cell lysate from Pseudomonas species for colony PCR reactions. The chapter continues with a switch in focus from DNA to RNA, describing methods for RNA isolation from Pseudomonas, cDNA generation, and finally q-RT-PCR to investigate relative changes in gene expression.

KW - CDNA

KW - Cloning

KW - Colony PCR

KW - Gene amplification

KW - PCR

KW - QRT-PCR

KW - RNA

KW - RT-PCR

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DO - 10.1007/978-1-4939-0473-0_35

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AN - SCOPUS:84927164372

SN - 1064-3745

VL - 1149

SP - 457

EP - 468

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Gene amplification and qRT-PCR

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

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