Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network

Jian Hui Low; Pin Li; Elaine Guo Yan Chew; Bingrui Zhou; Keiichiro Suzuki; Tian Zhang; Michelle Mulan Lian; Meng Liu; Emi Aizawa; Concepcion Rodriguez Esteban; Kylie Su Mei Yong; Qingfeng Chen; Josep M. Campistol; Mingliang Fang; Chiea Chuen Khor; Jia Nee Foo; Juan Carlos Izpisua Belmonte; Yun Xia

doi:10.1016/j.stem.2019.06.009

Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network

Jian Hui Low, Pin Li, Elaine Guo Yan Chew, Bingrui Zhou, Keiichiro Suzuki, Tian Zhang, Michelle Mulan Lian, Meng Liu, Emi Aizawa, Concepcion Rodriguez Esteban, Kylie Su Mei Yong, Qingfeng Chen, Josep M. Campistol, Mingliang Fang, Chiea Chuen Khor, Jia Nee Foo^*, Juan Carlos Izpisua Belmonte, Yun Xia

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

259 Citations (Scopus)

Abstract

Human PSC-derived organoids are an amenable platform for understanding human development and diseases despite numerous limitations. Low et al. establish a versatile platform for generating vascularized and patterned kidney organoids. Using this platform, they identified a non-conventional origin of renal vasculature and recapitulated ARPKD cystogenesis in vitro.

Original language	English
Pages (from-to)	373-387.e9
Journal	Cell Stem Cell
Volume	25
Issue number	3
DOIs	https://doi.org/10.1016/j.stem.2019.06.009
Publication status	Published - Sept 5 2019
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2019 Elsevier Inc.

ASJC Scopus Subject Areas

Molecular Medicine
Genetics
Cell Biology

Keywords

ARPKD
developmental modeling
disease modeling
implantation
kidney organoid
nephron patterning
patient-specific iPSC
pluripotent stem cells
single-cell RNA-seq
vascular progenitors

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.stem.2019.06.009

Cite this

Low, J. H., Li, P., Chew, E. G. Y., Zhou, B., Suzuki, K., Zhang, T., Lian, M. M., Liu, M., Aizawa, E., Rodriguez Esteban, C., Yong, K. S. M., Chen, Q., Campistol, J. M., Fang, M., Khor, C. C., Foo, J. N., Izpisua Belmonte, J. C., & Xia, Y. (2019). Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network. Cell Stem Cell, 25(3), 373-387.e9. https://doi.org/10.1016/j.stem.2019.06.009

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title = "Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network",

abstract = "Human PSC-derived organoids are an amenable platform for understanding human development and diseases despite numerous limitations. Low et al. establish a versatile platform for generating vascularized and patterned kidney organoids. Using this platform, they identified a non-conventional origin of renal vasculature and recapitulated ARPKD cystogenesis in vitro.",

keywords = "ARPKD, developmental modeling, disease modeling, implantation, kidney organoid, nephron patterning, patient-specific iPSC, pluripotent stem cells, single-cell RNA-seq, vascular progenitors",

author = "Low, {Jian Hui} and Pin Li and Chew, {Elaine Guo Yan} and Bingrui Zhou and Keiichiro Suzuki and Tian Zhang and Lian, {Michelle Mulan} and Meng Liu and Emi Aizawa and {Rodriguez Esteban}, Concepcion and Yong, {Kylie Su Mei} and Qingfeng Chen and Campistol, {Josep M.} and Mingliang Fang and Khor, {Chiea Chuen} and Foo, {Jia Nee} and {Izpisua Belmonte}, {Juan Carlos} and Yun Xia",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",

year = "2019",

month = sep,

day = "5",

doi = "10.1016/j.stem.2019.06.009",

language = "English",

volume = "25",

pages = "373--387.e9",

journal = "Cell Stem Cell",

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Low, JH, Li, P, Chew, EGY, Zhou, B, Suzuki, K, Zhang, T, Lian, MM, Liu, M, Aizawa, E, Rodriguez Esteban, C, Yong, KSM, Chen, Q, Campistol, JM, Fang, M, Khor, CC, Foo, JN, Izpisua Belmonte, JC & Xia, Y 2019, 'Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network', Cell Stem Cell, vol. 25, no. 3, pp. 373-387.e9. https://doi.org/10.1016/j.stem.2019.06.009

TY - JOUR

T1 - Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network

AU - Low, Jian Hui

AU - Li, Pin

AU - Chew, Elaine Guo Yan

AU - Zhou, Bingrui

AU - Suzuki, Keiichiro

AU - Zhang, Tian

AU - Lian, Michelle Mulan

AU - Liu, Meng

AU - Aizawa, Emi

AU - Rodriguez Esteban, Concepcion

AU - Yong, Kylie Su Mei

AU - Chen, Qingfeng

AU - Campistol, Josep M.

AU - Fang, Mingliang

AU - Khor, Chiea Chuen

AU - Foo, Jia Nee

AU - Izpisua Belmonte, Juan Carlos

AU - Xia, Yun

PY - 2019/9/5

Y1 - 2019/9/5

N2 - Human PSC-derived organoids are an amenable platform for understanding human development and diseases despite numerous limitations. Low et al. establish a versatile platform for generating vascularized and patterned kidney organoids. Using this platform, they identified a non-conventional origin of renal vasculature and recapitulated ARPKD cystogenesis in vitro.

AB - Human PSC-derived organoids are an amenable platform for understanding human development and diseases despite numerous limitations. Low et al. establish a versatile platform for generating vascularized and patterned kidney organoids. Using this platform, they identified a non-conventional origin of renal vasculature and recapitulated ARPKD cystogenesis in vitro.

KW - ARPKD

KW - developmental modeling

KW - disease modeling

KW - implantation

KW - kidney organoid

KW - nephron patterning

KW - patient-specific iPSC

KW - pluripotent stem cells

KW - single-cell RNA-seq

KW - vascular progenitors

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UR - http://www.scopus.com/inward/citedby.url?scp=85071595433&partnerID=8YFLogxK

U2 - 10.1016/j.stem.2019.06.009

DO - 10.1016/j.stem.2019.06.009

M3 - Article

C2 - 31303547

AN - SCOPUS:85071595433

SN - 1934-5909

VL - 25

SP - 373-387.e9

JO - Cell Stem Cell

JF - Cell Stem Cell

IS - 3

ER -

Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

UN SDGs

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