Genetic improvement of Magnetospirillum gryphiswaldense for enhanced biological removal of phosphate

Yanping Zhou; Wiktor Lisowski; Yan Zhou; Ng Wun Jern; Kama Huang; Eileen Fong

doi:10.1007/s10529-017-2383-5

Genetic improvement of Magnetospirillum gryphiswaldense for enhanced biological removal of phosphate

Yanping Zhou, Wiktor Lisowski, Yan Zhou, Ng Wun Jern, Kama Huang, Eileen Fong^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Objectives: To improve its phosphate accumulating abilities for phosphate recycling from wastewater, a magnetotactic bacterium, Magnetospirillum gryphiswaldense, was genetically modified to over-express polyphosphate kinase. Results: Polyphosphate kinase was over-expressed in the bacterium. The recombinant strain accumulated ninefold more polyphosphate from synthetic wastewater compared to original wild type. The magnetic property of the recombinant M. gryphiswaldense strain was retained. Conclusions: The recombinant M. gryphiswaldense can be used for phosphate removal and recovery in bioremediation.

Original language	English
Pages (from-to)	1509-1514
Number of pages	6
Journal	Biotechnology Letters
Volume	39
Issue number	10
DOIs	https://doi.org/10.1007/s10529-017-2383-5
Publication status	Published - Oct 1 2017
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media B.V.

ASJC Scopus Subject Areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Keywords

Biological phosphate removal
Genetic engineering
Magnetic bacteria
Magnetospirillum
Phosphate removal

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10.1007/s10529-017-2383-5

Cite this

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title = "Genetic improvement of Magnetospirillum gryphiswaldense for enhanced biological removal of phosphate",

abstract = "Objectives: To improve its phosphate accumulating abilities for phosphate recycling from wastewater, a magnetotactic bacterium, Magnetospirillum gryphiswaldense, was genetically modified to over-express polyphosphate kinase. Results: Polyphosphate kinase was over-expressed in the bacterium. The recombinant strain accumulated ninefold more polyphosphate from synthetic wastewater compared to original wild type. The magnetic property of the recombinant M. gryphiswaldense strain was retained. Conclusions: The recombinant M. gryphiswaldense can be used for phosphate removal and recovery in bioremediation.",

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author = "Yanping Zhou and Wiktor Lisowski and Yan Zhou and Jern, {Ng Wun} and Kama Huang and Eileen Fong",

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T1 - Genetic improvement of Magnetospirillum gryphiswaldense for enhanced biological removal of phosphate

AU - Zhou, Yanping

AU - Lisowski, Wiktor

AU - Zhou, Yan

AU - Jern, Ng Wun

AU - Huang, Kama

AU - Fong, Eileen

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Objectives: To improve its phosphate accumulating abilities for phosphate recycling from wastewater, a magnetotactic bacterium, Magnetospirillum gryphiswaldense, was genetically modified to over-express polyphosphate kinase. Results: Polyphosphate kinase was over-expressed in the bacterium. The recombinant strain accumulated ninefold more polyphosphate from synthetic wastewater compared to original wild type. The magnetic property of the recombinant M. gryphiswaldense strain was retained. Conclusions: The recombinant M. gryphiswaldense can be used for phosphate removal and recovery in bioremediation.

AB - Objectives: To improve its phosphate accumulating abilities for phosphate recycling from wastewater, a magnetotactic bacterium, Magnetospirillum gryphiswaldense, was genetically modified to over-express polyphosphate kinase. Results: Polyphosphate kinase was over-expressed in the bacterium. The recombinant strain accumulated ninefold more polyphosphate from synthetic wastewater compared to original wild type. The magnetic property of the recombinant M. gryphiswaldense strain was retained. Conclusions: The recombinant M. gryphiswaldense can be used for phosphate removal and recovery in bioremediation.

KW - Biological phosphate removal

KW - Genetic engineering

KW - Magnetic bacteria

KW - Magnetospirillum

KW - Phosphate removal

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Genetic improvement of Magnetospirillum gryphiswaldense for enhanced biological removal of phosphate

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

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