TY - JOUR
T1 - Genomic DNA fingerprinting of clinical isolates of Helicobacter pylori by REP-PCR and restriction fragment end-labelling
AU - Van Doorn, Nathalie E.M.
AU - Namavar, Ferry
AU - Kusters, Johannes G.
AU - Van Rees, Emmelien P.
AU - Kuipers, Ernst J.
AU - De Graaff, Johannes
PY - 1998/3/1
Y1 - 1998/3/1
N2 - Generic diversity of 32 Helicobacter pylori strains isolated from patients with gastritis, gastric or duodenal ulcer, carcinoma, or lymphoma was determined by repetitive sequence element polymerase chain reaction (REP-PCR), and by the new typing method restriction fragment end-labelling (RFEL). Furthermore, these two methods were used to investigate a possible correlation between clinical symptoms and the genetic background of Helicobacter pylori. Both REP-PCR and RFEL revealed 31 different patterns for the 32 strains tested, but the pair of isolates with identical REP-PCR patterns was not the same as the pair of isolates with identical RFEL patterns. Computer-assisted analysis of the DNA fingerprints was used to determine similarity coefficients. This analysis revealed no clustering of disease-specific strains by any of the two methods.
AB - Generic diversity of 32 Helicobacter pylori strains isolated from patients with gastritis, gastric or duodenal ulcer, carcinoma, or lymphoma was determined by repetitive sequence element polymerase chain reaction (REP-PCR), and by the new typing method restriction fragment end-labelling (RFEL). Furthermore, these two methods were used to investigate a possible correlation between clinical symptoms and the genetic background of Helicobacter pylori. Both REP-PCR and RFEL revealed 31 different patterns for the 32 strains tested, but the pair of isolates with identical REP-PCR patterns was not the same as the pair of isolates with identical RFEL patterns. Computer-assisted analysis of the DNA fingerprints was used to determine similarity coefficients. This analysis revealed no clustering of disease-specific strains by any of the two methods.
KW - Cluster analysis
KW - Gastric disease
KW - Genotyping
KW - Helicobacter pylori
UR - http://www.scopus.com/inward/record.url?scp=0032032896&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032032896&partnerID=8YFLogxK
U2 - 10.1016/S0378-1097(98)00025-1
DO - 10.1016/S0378-1097(98)00025-1
M3 - Article
C2 - 9495025
AN - SCOPUS:0032032896
SN - 0378-1097
VL - 160
SP - 145
EP - 150
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -