Gold Nanoparticle Smartphone Platform for Diagnosing Urinary Tract Infections

Kyryl Zagorovsky; Maria Teresa Fernández-Argüelles; Diane Bona; Ashraf Mohamed Elshawadfy; Abdullah Muhammad Syed; Pranav Kadhiresan; Tony Mazzulli; Karen L. Maxwell; Warren C.W. Chan

doi:10.1021/acsnanoscienceau.2c00001

Gold Nanoparticle Smartphone Platform for Diagnosing Urinary Tract Infections

Kyryl Zagorovsky, Maria Teresa Fernández-Argüelles, Diane Bona, Ashraf Mohamed Elshawadfy, Abdullah Muhammad Syed, Pranav Kadhiresan, Tony Mazzulli, Karen L. Maxwell, Warren C.W. Chan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Current urinary tract infection (UTI) diagnostic methods are slow or provide limited information, resulting in prescribing antibiotic therapy before bacterial pathogen identification. Here, we adapted a gold nanoparticle colorimetric approach and developed a smartphone platform for UTI detection. We show the parallel identification of five major UTI pathogens at clinically relevant concentrations of 10⁵bacteria/mL using bacteria-specific and universal probes. We validated the diagnostic technology using 115 positive and 19 negative samples from patients with Escherichia coli, Proteus mirabilis, and Klebsiella pneumoniae infections. The assay successfully identified the infecting pathogen (specificity: >98% and sensitivity: 51-73%) in 3 h. Our platform is faster than culturing and can wirelessly store and transmit results at the cost of $0.38 per assay.

Original language	English
Pages (from-to)	324-332
Number of pages	9
Journal	ACS Nanoscience Au
Volume	2
Issue number	4
DOIs	https://doi.org/10.1021/acsnanoscienceau.2c00001
Publication status	Published - Aug 17 2022
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2022 by the Author(s).

ASJC Scopus Subject Areas

Chemistry (miscellaneous)
Materials Science (miscellaneous)

Keywords

diagnostics
DNAzymes
gold nanoparticles
point of care device
smartphone
urinary tract infection

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10.1021/acsnanoscienceau.2c00001

Cite this

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title = "Gold Nanoparticle Smartphone Platform for Diagnosing Urinary Tract Infections",

abstract = "Current urinary tract infection (UTI) diagnostic methods are slow or provide limited information, resulting in prescribing antibiotic therapy before bacterial pathogen identification. Here, we adapted a gold nanoparticle colorimetric approach and developed a smartphone platform for UTI detection. We show the parallel identification of five major UTI pathogens at clinically relevant concentrations of 105bacteria/mL using bacteria-specific and universal probes. We validated the diagnostic technology using 115 positive and 19 negative samples from patients with Escherichia coli, Proteus mirabilis, and Klebsiella pneumoniae infections. The assay successfully identified the infecting pathogen (specificity: >98% and sensitivity: 51-73%) in 3 h. Our platform is faster than culturing and can wirelessly store and transmit results at the cost of $0.38 per assay.",

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AU - Zagorovsky, Kyryl

AU - Fernández-Argüelles, Maria Teresa

AU - Bona, Diane

AU - Elshawadfy, Ashraf Mohamed

AU - Syed, Abdullah Muhammad

AU - Kadhiresan, Pranav

AU - Mazzulli, Tony

AU - Maxwell, Karen L.

AU - Chan, Warren C.W.

PY - 2022/8/17

Y1 - 2022/8/17

N2 - Current urinary tract infection (UTI) diagnostic methods are slow or provide limited information, resulting in prescribing antibiotic therapy before bacterial pathogen identification. Here, we adapted a gold nanoparticle colorimetric approach and developed a smartphone platform for UTI detection. We show the parallel identification of five major UTI pathogens at clinically relevant concentrations of 105bacteria/mL using bacteria-specific and universal probes. We validated the diagnostic technology using 115 positive and 19 negative samples from patients with Escherichia coli, Proteus mirabilis, and Klebsiella pneumoniae infections. The assay successfully identified the infecting pathogen (specificity: >98% and sensitivity: 51-73%) in 3 h. Our platform is faster than culturing and can wirelessly store and transmit results at the cost of $0.38 per assay.

AB - Current urinary tract infection (UTI) diagnostic methods are slow or provide limited information, resulting in prescribing antibiotic therapy before bacterial pathogen identification. Here, we adapted a gold nanoparticle colorimetric approach and developed a smartphone platform for UTI detection. We show the parallel identification of five major UTI pathogens at clinically relevant concentrations of 105bacteria/mL using bacteria-specific and universal probes. We validated the diagnostic technology using 115 positive and 19 negative samples from patients with Escherichia coli, Proteus mirabilis, and Klebsiella pneumoniae infections. The assay successfully identified the infecting pathogen (specificity: >98% and sensitivity: 51-73%) in 3 h. Our platform is faster than culturing and can wirelessly store and transmit results at the cost of $0.38 per assay.

KW - diagnostics

KW - DNAzymes

KW - gold nanoparticles

KW - point of care device

KW - smartphone

KW - urinary tract infection

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Gold Nanoparticle Smartphone Platform for Diagnosing Urinary Tract Infections

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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