Hepatitis B virus X protein in the proteasome of mammalian cells: Defining the targeting domain

Wei Ning Chen; Chong Jin Oon; Kian Sim Goo

doi:10.1023/A:1011997704112

Hepatitis B virus X protein in the proteasome of mammalian cells: Defining the targeting domain

Wei Ning Chen^*, Chong Jin Oon, Kian Sim Goo

^*Corresponding author for this work

Singapore General Hospital

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The hepatitis B virus X (HBX) protein has been implicated in both hepatitis B virus-related pathogenesis and also in diverse cellular processes. The diversity of its activities may be mediated through its interaction with cellular organelles. However no clearly defined subcellular localization of HBX is available. We report here the localization of HBX in the proteasome complexes using green fluorescent protein tag. A new proteasome-targeting domain has also been defined in HBX by deletion study. Furthermore, a functional role of HBX in the cellular processes mediated by the proteasome complexes has been suggested by its cell cycle-independent localization in the proteasome. Further analysis of the functional role of HBX in the proteasome complexes should provide more information on the underlying mechanism of HBX activities.

Original language	English
Pages (from-to)	31-34
Number of pages	4
Journal	Molecular Biology Reports
Volume	28
Issue number	1
DOIs	https://doi.org/10.1023/A:1011997704112
Publication status	Published - 2001
Externally published	Yes

ASJC Scopus Subject Areas

Molecular Biology
Genetics

Keywords

Deletion analysis
Green fluorescent protein
Hepatitis B virus
Localization
Proteasome
X protein

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1023/A:1011997704112

Cite this

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title = "Hepatitis B virus X protein in the proteasome of mammalian cells: Defining the targeting domain",

abstract = "The hepatitis B virus X (HBX) protein has been implicated in both hepatitis B virus-related pathogenesis and also in diverse cellular processes. The diversity of its activities may be mediated through its interaction with cellular organelles. However no clearly defined subcellular localization of HBX is available. We report here the localization of HBX in the proteasome complexes using green fluorescent protein tag. A new proteasome-targeting domain has also been defined in HBX by deletion study. Furthermore, a functional role of HBX in the cellular processes mediated by the proteasome complexes has been suggested by its cell cycle-independent localization in the proteasome. Further analysis of the functional role of HBX in the proteasome complexes should provide more information on the underlying mechanism of HBX activities.",

keywords = "Deletion analysis, Green fluorescent protein, Hepatitis B virus, Localization, Proteasome, X protein",

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T1 - Hepatitis B virus X protein in the proteasome of mammalian cells

T2 - Defining the targeting domain

AU - Chen, Wei Ning

AU - Oon, Chong Jin

AU - Goo, Kian Sim

PY - 2001

Y1 - 2001

N2 - The hepatitis B virus X (HBX) protein has been implicated in both hepatitis B virus-related pathogenesis and also in diverse cellular processes. The diversity of its activities may be mediated through its interaction with cellular organelles. However no clearly defined subcellular localization of HBX is available. We report here the localization of HBX in the proteasome complexes using green fluorescent protein tag. A new proteasome-targeting domain has also been defined in HBX by deletion study. Furthermore, a functional role of HBX in the cellular processes mediated by the proteasome complexes has been suggested by its cell cycle-independent localization in the proteasome. Further analysis of the functional role of HBX in the proteasome complexes should provide more information on the underlying mechanism of HBX activities.

AB - The hepatitis B virus X (HBX) protein has been implicated in both hepatitis B virus-related pathogenesis and also in diverse cellular processes. The diversity of its activities may be mediated through its interaction with cellular organelles. However no clearly defined subcellular localization of HBX is available. We report here the localization of HBX in the proteasome complexes using green fluorescent protein tag. A new proteasome-targeting domain has also been defined in HBX by deletion study. Furthermore, a functional role of HBX in the cellular processes mediated by the proteasome complexes has been suggested by its cell cycle-independent localization in the proteasome. Further analysis of the functional role of HBX in the proteasome complexes should provide more information on the underlying mechanism of HBX activities.

KW - Deletion analysis

KW - Green fluorescent protein

KW - Hepatitis B virus

KW - Localization

KW - Proteasome

KW - X protein

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Hepatitis B virus X protein in the proteasome of mammalian cells: Defining the targeting domain

Abstract

ASJC Scopus Subject Areas

Keywords

UN SDGs

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