High phylogenetic diversity of transconjugants carrying plasmid pJP4 in an activated sludge-derived microbial community

Stephan Bathe; Michael Lebuhn; Joachim W. Ellwart; Stefan Wuertz; Martina Hausner

doi:10.1016/j.femsle.2004.04.038

High phylogenetic diversity of transconjugants carrying plasmid pJP4 in an activated sludge-derived microbial community

Stephan Bathe, Michael Lebuhn, Joachim W. Ellwart, Stefan Wuertz, Martina Hausner^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Transconjugants of plasmid pJP4, originating from an agar plate mating of a Pseudomonas putida donor with an activated sludge-derived microbial community, were isolated and identified by partial 16S rDNA sequencing. The transconjugant strains belonged to a variety of genera of the α-, β-, γβ- and γ-classes of the Proteobacteria, mostly to the families Rhizobiaceae and Comamonadaceae and the genus Stenotrophomonas. Only P. putida and Delftia spp. strains were able to grow on 2,4-D as the sole carbon source.

Original language	English
Pages (from-to)	215-219
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	235
Issue number	2
DOIs	https://doi.org/10.1016/j.femsle.2004.04.038
Publication status	Published - Jun 15 2004
Externally published	Yes

ASJC Scopus Subject Areas

General Medicine

Keywords

Activated sludge
Catabolic plasmid
Conjugation
Fluorescent protein
Horizontal gene transfer
Host range
pJP4

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10.1016/j.femsle.2004.04.038

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title = "High phylogenetic diversity of transconjugants carrying plasmid pJP4 in an activated sludge-derived microbial community",

abstract = "Transconjugants of plasmid pJP4, originating from an agar plate mating of a Pseudomonas putida donor with an activated sludge-derived microbial community, were isolated and identified by partial 16S rDNA sequencing. The transconjugant strains belonged to a variety of genera of the α-, β-, γβ- and γ-classes of the Proteobacteria, mostly to the families Rhizobiaceae and Comamonadaceae and the genus Stenotrophomonas. Only P. putida and Delftia spp. strains were able to grow on 2,4-D as the sole carbon source.",

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T1 - High phylogenetic diversity of transconjugants carrying plasmid pJP4 in an activated sludge-derived microbial community

AU - Bathe, Stephan

AU - Lebuhn, Michael

AU - Ellwart, Joachim W.

AU - Wuertz, Stefan

AU - Hausner, Martina

PY - 2004/6/15

Y1 - 2004/6/15

N2 - Transconjugants of plasmid pJP4, originating from an agar plate mating of a Pseudomonas putida donor with an activated sludge-derived microbial community, were isolated and identified by partial 16S rDNA sequencing. The transconjugant strains belonged to a variety of genera of the α-, β-, γβ- and γ-classes of the Proteobacteria, mostly to the families Rhizobiaceae and Comamonadaceae and the genus Stenotrophomonas. Only P. putida and Delftia spp. strains were able to grow on 2,4-D as the sole carbon source.

AB - Transconjugants of plasmid pJP4, originating from an agar plate mating of a Pseudomonas putida donor with an activated sludge-derived microbial community, were isolated and identified by partial 16S rDNA sequencing. The transconjugant strains belonged to a variety of genera of the α-, β-, γβ- and γ-classes of the Proteobacteria, mostly to the families Rhizobiaceae and Comamonadaceae and the genus Stenotrophomonas. Only P. putida and Delftia spp. strains were able to grow on 2,4-D as the sole carbon source.

KW - Activated sludge

KW - Catabolic plasmid

KW - Conjugation

KW - Fluorescent protein

KW - Horizontal gene transfer

KW - Host range

KW - pJP4

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High phylogenetic diversity of transconjugants carrying plasmid pJP4 in an activated sludge-derived microbial community

Abstract

ASJC Scopus Subject Areas

Keywords

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