High rates of conjugation in bacterial biofilms as determined by quantitative in situ analysis

Martina Hausner; Stefan Wuertz

doi:10.1128/aem.65.8.3710-3713.1999

High rates of conjugation in bacterial biofilms as determined by quantitative in situ analysis

Martina Hausner, Stefan Wuertz^*

^*Corresponding author for this work

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

383 Citations (Scopus)

Abstract

Quantitative in situ determination of conjugative gene transfer in defined bacterial biofilms using automated confocal laser scanning microscopy followed by three-dimensional analysis of cellular biovolumes revealed conjugation rates 1,000-fold higher than those determined by classical plating techniques. Conjugation events were not affected by nutrient concentration alone but were influenced by time and biofilm structure.

Original language	English
Pages (from-to)	3710-3713
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	65
Issue number	8
DOIs	https://doi.org/10.1128/aem.65.8.3710-3713.1999
Publication status	Published - Aug 1999
Externally published	Yes

ASJC Scopus Subject Areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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10.1128/aem.65.8.3710-3713.1999

Cite this

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title = "High rates of conjugation in bacterial biofilms as determined by quantitative in situ analysis",

abstract = "Quantitative in situ determination of conjugative gene transfer in defined bacterial biofilms using automated confocal laser scanning microscopy followed by three-dimensional analysis of cellular biovolumes revealed conjugation rates 1,000-fold higher than those determined by classical plating techniques. Conjugation events were not affected by nutrient concentration alone but were influenced by time and biofilm structure.",

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doi = "10.1128/aem.65.8.3710-3713.1999",

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AB - Quantitative in situ determination of conjugative gene transfer in defined bacterial biofilms using automated confocal laser scanning microscopy followed by three-dimensional analysis of cellular biovolumes revealed conjugation rates 1,000-fold higher than those determined by classical plating techniques. Conjugation events were not affected by nutrient concentration alone but were influenced by time and biofilm structure.

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AN - SCOPUS:0032766408

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High rates of conjugation in bacterial biofilms as determined by quantitative in situ analysis

Abstract

ASJC Scopus Subject Areas

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