Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry

Yong Mei Hu; Yi Tao Wang; Stephen C.W. Sze; K. W. Tsang; H. K. Wong; Qing Liu; Li Dan Zhong; Yao Tong

doi:10.1002/bmc.1315

Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry

Yong Mei Hu, Yi Tao Wang, Stephen C.W. Sze, K. W. Tsang, H. K. Wong, Qing Liu, Li Dan Zhong, Yao Tong^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

A simple and specific LC-DAD-ESI-MS/MS method has been developed and applied for the primary investigation of the chemical constituents absorbed or metabolized in vivo, after the rat oral administration of Erxian Decoction (EXD), a Chinese medicine prescription for menopausal syndromes. Through the online ESI-MSⁿ analysis, a total of 35 compounds have been identified or tentatively characterized from the seven tested samples, and 13 of them were unambiguously identified through a direct comparison of the retention time, UV spectra and MSⁿ fragmentation patterns with the authentic ones. The results showed that 21 compounds were detected from rat plasma, 20 compounds were detected from rat kidneys and adrenal glands, 19 compounds were detected from rat ovaries, 12 compounds were found in rat intestines, nine compounds were identified from rat livers and nine compounds were detected from rat brains at certain time points after oral administration of the eff ective EXD fraction.

Original language	English
Pages (from-to)	479-489
Number of pages	11
Journal	Biomedical Chromatography
Volume	24
Issue number	5
DOIs	https://doi.org/10.1002/bmc.1315
Publication status	Published - May 2010
Externally published	Yes

ASJC Scopus Subject Areas

Analytical Chemistry
Biochemistry
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

Keywords

Erxian Decoction (EXD)
LC-DAD-ESI-MS
Menopausal syndromes
Metabolites
Pharmacokinetic study
Traditional Chinese medicine prescriptions (TCMP)

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Hu, Y. M., Wang, Y. T., Sze, S. C. W., Tsang, K. W., Wong, H. K., Liu, Q., Zhong, L. D., & Tong, Y. (2010). Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry. Biomedical Chromatography, 24(5), 479-489. https://doi.org/10.1002/bmc.1315

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title = "Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry",

abstract = "A simple and specific LC-DAD-ESI-MS/MS method has been developed and applied for the primary investigation of the chemical constituents absorbed or metabolized in vivo, after the rat oral administration of Erxian Decoction (EXD), a Chinese medicine prescription for menopausal syndromes. Through the online ESI-MSn analysis, a total of 35 compounds have been identified or tentatively characterized from the seven tested samples, and 13 of them were unambiguously identified through a direct comparison of the retention time, UV spectra and MSn fragmentation patterns with the authentic ones. The results showed that 21 compounds were detected from rat plasma, 20 compounds were detected from rat kidneys and adrenal glands, 19 compounds were detected from rat ovaries, 12 compounds were found in rat intestines, nine compounds were identified from rat livers and nine compounds were detected from rat brains at certain time points after oral administration of the eff ective EXD fraction.",

keywords = "Erxian Decoction (EXD), LC-DAD-ESI-MS, Menopausal syndromes, Metabolites, Pharmacokinetic study, Traditional Chinese medicine prescriptions (TCMP)",

author = "Hu, {Yong Mei} and Wang, {Yi Tao} and Sze, {Stephen C.W.} and Tsang, {K. W.} and Wong, {H. K.} and Qing Liu and Zhong, {Li Dan} and Yao Tong",

year = "2010",

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doi = "10.1002/bmc.1315",

language = "English",

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pages = "479--489",

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Hu, YM, Wang, YT, Sze, SCW, Tsang, KW, Wong, HK, Liu, Q, Zhong, LD & Tong, Y 2010, 'Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry', Biomedical Chromatography, vol. 24, no. 5, pp. 479-489. https://doi.org/10.1002/bmc.1315

Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry. / Hu, Yong Mei; Wang, Yi Tao; Sze, Stephen C.W. et al.
In: Biomedical Chromatography, Vol. 24, No. 5, 05.2010, p. 479-489.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

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AU - Hu, Yong Mei

AU - Wang, Yi Tao

AU - Sze, Stephen C.W.

AU - Tsang, K. W.

AU - Wong, H. K.

AU - Liu, Qing

AU - Zhong, Li Dan

AU - Tong, Yao

PY - 2010/5

Y1 - 2010/5

N2 - A simple and specific LC-DAD-ESI-MS/MS method has been developed and applied for the primary investigation of the chemical constituents absorbed or metabolized in vivo, after the rat oral administration of Erxian Decoction (EXD), a Chinese medicine prescription for menopausal syndromes. Through the online ESI-MSn analysis, a total of 35 compounds have been identified or tentatively characterized from the seven tested samples, and 13 of them were unambiguously identified through a direct comparison of the retention time, UV spectra and MSn fragmentation patterns with the authentic ones. The results showed that 21 compounds were detected from rat plasma, 20 compounds were detected from rat kidneys and adrenal glands, 19 compounds were detected from rat ovaries, 12 compounds were found in rat intestines, nine compounds were identified from rat livers and nine compounds were detected from rat brains at certain time points after oral administration of the eff ective EXD fraction.

AB - A simple and specific LC-DAD-ESI-MS/MS method has been developed and applied for the primary investigation of the chemical constituents absorbed or metabolized in vivo, after the rat oral administration of Erxian Decoction (EXD), a Chinese medicine prescription for menopausal syndromes. Through the online ESI-MSn analysis, a total of 35 compounds have been identified or tentatively characterized from the seven tested samples, and 13 of them were unambiguously identified through a direct comparison of the retention time, UV spectra and MSn fragmentation patterns with the authentic ones. The results showed that 21 compounds were detected from rat plasma, 20 compounds were detected from rat kidneys and adrenal glands, 19 compounds were detected from rat ovaries, 12 compounds were found in rat intestines, nine compounds were identified from rat livers and nine compounds were detected from rat brains at certain time points after oral administration of the eff ective EXD fraction.

KW - Erxian Decoction (EXD)

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KW - Metabolites

KW - Pharmacokinetic study

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ER -

Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry

Abstract

ASJC Scopus Subject Areas

Keywords

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