TY - GEN
T1 - Immobilization of bovine serum albumin on self-assembled monolayer modified dielectric film for biosensor application
AU - Tan, Ooi Kiang
AU - Fang, Xiangyi
AU - Wei, Qiu
AU - Yao, Manwen
AU - Tjin, S. C.
PY - 2005
Y1 - 2005
N2 - For the first time, the Sol-Gel derived dielectric barium strontium titanate (BST) film has been explored as a transducer material for bio-sensor. Self-assembled monolayer (SAM) of mono-dodecyl phosphate (DDPO4) has been coated on the BST film from aqueous solution of the alkyl phosphate ammonium salts, resulting in a highly hydrophobic surface with water contact angle of 99.8° compared to the original contact angle of 40.8° for the bare BST surface. The microscopic features and the bio-sensing properties were characterized using atomic force microscopy (AFM), electrochemical impedance spectroscopy (EIS) in the presence of [Fe(CN)6]3-/4- as a redox couple. AFM images revealed the well immobilized BSA on the SAM modified BST surface. The EIS results showed significant increase in the electron transfer resistance compared to all other electrical elements in the equivalent circuit. Specific binding of anti-BSA on the immobilized BSA surface was observed by AFM imaging and EIS measurements. It was found that the specific binding of anti-BSA can be sensitively detected by measuring the electron transfer resistance. The present investigation indicates the potential of dielectric BST for electrochemical biosensor applications.
AB - For the first time, the Sol-Gel derived dielectric barium strontium titanate (BST) film has been explored as a transducer material for bio-sensor. Self-assembled monolayer (SAM) of mono-dodecyl phosphate (DDPO4) has been coated on the BST film from aqueous solution of the alkyl phosphate ammonium salts, resulting in a highly hydrophobic surface with water contact angle of 99.8° compared to the original contact angle of 40.8° for the bare BST surface. The microscopic features and the bio-sensing properties were characterized using atomic force microscopy (AFM), electrochemical impedance spectroscopy (EIS) in the presence of [Fe(CN)6]3-/4- as a redox couple. AFM images revealed the well immobilized BSA on the SAM modified BST surface. The EIS results showed significant increase in the electron transfer resistance compared to all other electrical elements in the equivalent circuit. Specific binding of anti-BSA on the immobilized BSA surface was observed by AFM imaging and EIS measurements. It was found that the specific binding of anti-BSA can be sensitively detected by measuring the electron transfer resistance. The present investigation indicates the potential of dielectric BST for electrochemical biosensor applications.
UR - http://www.scopus.com/inward/record.url?scp=33847278944&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33847278944&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2005.1597719
DO - 10.1109/ICSENS.2005.1597719
M3 - Conference contribution
AN - SCOPUS:33847278944
SN - 0780390563
SN - 9780780390560
T3 - Proceedings of IEEE Sensors
SP - 397
EP - 400
BT - Proceedings of the Fourth IEEE Conference on Sensors 2005
T2 - Fourth IEEE Conference on Sensors 2005
Y2 - 31 October 2005 through 3 November 2005
ER -