Immunometabolomic phenotyping of motile T-cells

Madhavi Latha Somaraju Chalasani; Gaelin Looi Wen Dong; Navin Kumar Verma

doi:10.1007/978-1-4939-9036-8_12

Immunometabolomic phenotyping of motile T-cells

Madhavi Latha Somaraju Chalasani^*, Gaelin Looi Wen Dong, Navin Kumar Verma

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The immune system and its components defend our body against diverse pathogens and help in maintaining tissue homeostasis. Immune cells are highly dynamic in terms of their growth, migration, differentiation, and effector functions, and adopt diverse metabolic configurations to respond to varying immunological challenges. Growing body of evidence suggests that metabolic pathways fuel immune cells for their functioning, including T-cell migration to the site of infection. This chapter provides detailed methodology for the efficient extraction of T-cell metabolites for successful downstream immunometabolomic profiling of motile T-lymphocytes.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	91-98
Number of pages	8
DOIs	https://doi.org/10.1007/978-1-4939-9036-8_12
Publication status	Published - 2019
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1930
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

Keywords

Immunometabolism
LFA-1
Mass-spectrometry
T-cell migration

Access to Document

10.1007/978-1-4939-9036-8_12

Cite this

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abstract = "The immune system and its components defend our body against diverse pathogens and help in maintaining tissue homeostasis. Immune cells are highly dynamic in terms of their growth, migration, differentiation, and effector functions, and adopt diverse metabolic configurations to respond to varying immunological challenges. Growing body of evidence suggests that metabolic pathways fuel immune cells for their functioning, including T-cell migration to the site of infection. This chapter provides detailed methodology for the efficient extraction of T-cell metabolites for successful downstream immunometabolomic profiling of motile T-lymphocytes.",

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doi = "10.1007/978-1-4939-9036-8\_12",

language = "English",

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AU - Verma, Navin Kumar

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N2 - The immune system and its components defend our body against diverse pathogens and help in maintaining tissue homeostasis. Immune cells are highly dynamic in terms of their growth, migration, differentiation, and effector functions, and adopt diverse metabolic configurations to respond to varying immunological challenges. Growing body of evidence suggests that metabolic pathways fuel immune cells for their functioning, including T-cell migration to the site of infection. This chapter provides detailed methodology for the efficient extraction of T-cell metabolites for successful downstream immunometabolomic profiling of motile T-lymphocytes.

AB - The immune system and its components defend our body against diverse pathogens and help in maintaining tissue homeostasis. Immune cells are highly dynamic in terms of their growth, migration, differentiation, and effector functions, and adopt diverse metabolic configurations to respond to varying immunological challenges. Growing body of evidence suggests that metabolic pathways fuel immune cells for their functioning, including T-cell migration to the site of infection. This chapter provides detailed methodology for the efficient extraction of T-cell metabolites for successful downstream immunometabolomic profiling of motile T-lymphocytes.

KW - Immunometabolism

KW - LFA-1

KW - Mass-spectrometry

KW - T-cell migration

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T3 - Methods in Molecular Biology

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Immunometabolomic phenotyping of motile T-cells

Abstract

Publication series

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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