Abstract
Nonlinear microscopy, including two-photon microscopy, requires pulsed lasers as light source. Typically, when choosing the appropriate pulsed laser for two-photon microscopy, the pulse repetition rate, pulse width, total power output, and output beam diameter are among the critical parameters which are often emphasised. Here, we demonstrate that the pulse shape, often overlooked, can have significant impact on the two-photon microscopy excitation efficiency and the effective signal brightness. We provide metrics to ease practical selection of pulsed laser sources for two-photon microscopy.
| Original language | English |
|---|---|
| Article number | 515123 |
| Pages (from-to) | 552-564 |
| Number of pages | 13 |
| Journal | Optics Continuum |
| Volume | 3 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2024 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2024 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.
ASJC Scopus Subject Areas
- Electrical and Electronic Engineering
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics