TY - GEN
T1 - Insights into piezophily from genetic studies on the deep-sea bacterium, Photobacterium profundum SS9
AU - El-Hajj, Ziad W.
AU - Allcock, David
AU - Tryfona, Theodora
AU - Lauro, Federico M.
AU - Sawyer, Lindsay
AU - Bartlett, Douglas H.
AU - Ferguson, Gail P.
PY - 2010/2
Y1 - 2010/2
N2 - The deep-sea bacterium, Photobacterium profundum SS9, has been adopted as a model organism to understand the molecular basis of cold-adapted high-pressure-loving (piezophilic) growth. Despite growing optimally at 28 MPa (15° C), P. profundum SS9 can grow over a wide range of pressures and temperatures. The ability to grow at atmospheric pressure has enabled a limited set of genetic tools to be developed, which has provided genetic insights into the mechanism of piezophilic growth in P. profundum SS9. This review focuses on how genetic studies have uncovered the importance of processes affecting the DNA and the bacterial cell envelope in the piezophilic growth of P. profundum SS9. In addition, a method was developed to assess quantitative piezophilic colony growth of P. profundum SS9 on solid agar. Future studies, using this methodology, could provide novel insights into the molecular basis of piezophilic, surface-attached growth.
AB - The deep-sea bacterium, Photobacterium profundum SS9, has been adopted as a model organism to understand the molecular basis of cold-adapted high-pressure-loving (piezophilic) growth. Despite growing optimally at 28 MPa (15° C), P. profundum SS9 can grow over a wide range of pressures and temperatures. The ability to grow at atmospheric pressure has enabled a limited set of genetic tools to be developed, which has provided genetic insights into the mechanism of piezophilic growth in P. profundum SS9. This review focuses on how genetic studies have uncovered the importance of processes affecting the DNA and the bacterial cell envelope in the piezophilic growth of P. profundum SS9. In addition, a method was developed to assess quantitative piezophilic colony growth of P. profundum SS9 on solid agar. Future studies, using this methodology, could provide novel insights into the molecular basis of piezophilic, surface-attached growth.
KW - Cell envelope
KW - DNA replication
KW - H-NS
KW - Piezophily
KW - RecD
UR - http://www.scopus.com/inward/record.url?scp=77949284153&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77949284153&partnerID=8YFLogxK
U2 - 10.1111/j.1749-6632.2009.05178.x
DO - 10.1111/j.1749-6632.2009.05178.x
M3 - Conference contribution
AN - SCOPUS:77949284153
SN - 9781573317719
T3 - Annals of the New York Academy of Sciences
SP - 143
EP - 148
BT - High-Pressure Bioscience and Biotechnology
PB - Blackwell Publishing Inc.
ER -