Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells

Haiyang Yu; Yuan Siang Lui; Sijing Xiong; Wen Shing Leong; Feng Wen; Himawan Nurkahfianto; Sravendra Rana; David Tai Leong; Kee Woei Ng; Lay Poh Tan

doi:10.1089/scd.2012.0160

Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells

Haiyang Yu, Yuan Siang Lui, Sijing Xiong, Wen Shing Leong, Feng Wen, Himawan Nurkahfianto, Sravendra Rana, David Tai Leong, Kee Woei Ng, Lay Poh Tan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

We report the establishment of a novel platform to induce myogenic differentiation of human mesenchymal stem cells (hMSCs) via focal adhesion (FA) modulation, giving insights into the role of FA on stem cell differentiation. Micropatterning of collagen type I on a polyacrylamide gel with a stiffness of 10.2kPa efficiently modulated elongated FA. This elongated FA profile preferentially recruited the β₃ integrin cluster and induced specific myogenic differentiation at both transcription and translation levels with expression of myosin heavy chain and α-sarcomeric actin. This was initiated with elongation of FA complexes that triggered the RhoA downstream signaling toward a myogenic lineage commitment. This study also illustrates how one could partially control myogenic differentiation outcomes of similar-shaped hMSCs by modulating FA morphology and distribution. This technology increases our toolkit choice for controlled differentiation in muscle engineering.

Original language	English
Pages (from-to)	136-147
Number of pages	12
Journal	Stem Cells and Development
Volume	22
Issue number	1
DOIs	https://doi.org/10.1089/scd.2012.0160
Publication status	Published - Jan 1 2013
Externally published	Yes

ASJC Scopus Subject Areas

Hematology
Developmental Biology
Cell Biology

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abstract = "We report the establishment of a novel platform to induce myogenic differentiation of human mesenchymal stem cells (hMSCs) via focal adhesion (FA) modulation, giving insights into the role of FA on stem cell differentiation. Micropatterning of collagen type I on a polyacrylamide gel with a stiffness of 10.2kPa efficiently modulated elongated FA. This elongated FA profile preferentially recruited the β3 integrin cluster and induced specific myogenic differentiation at both transcription and translation levels with expression of myosin heavy chain and α-sarcomeric actin. This was initiated with elongation of FA complexes that triggered the RhoA downstream signaling toward a myogenic lineage commitment. This study also illustrates how one could partially control myogenic differentiation outcomes of similar-shaped hMSCs by modulating FA morphology and distribution. This technology increases our toolkit choice for controlled differentiation in muscle engineering.",

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AU - Leong, Wen Shing

AU - Wen, Feng

AU - Nurkahfianto, Himawan

AU - Rana, Sravendra

AU - Leong, David Tai

AU - Ng, Kee Woei

AU - Tan, Lay Poh

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AB - We report the establishment of a novel platform to induce myogenic differentiation of human mesenchymal stem cells (hMSCs) via focal adhesion (FA) modulation, giving insights into the role of FA on stem cell differentiation. Micropatterning of collagen type I on a polyacrylamide gel with a stiffness of 10.2kPa efficiently modulated elongated FA. This elongated FA profile preferentially recruited the β3 integrin cluster and induced specific myogenic differentiation at both transcription and translation levels with expression of myosin heavy chain and α-sarcomeric actin. This was initiated with elongation of FA complexes that triggered the RhoA downstream signaling toward a myogenic lineage commitment. This study also illustrates how one could partially control myogenic differentiation outcomes of similar-shaped hMSCs by modulating FA morphology and distribution. This technology increases our toolkit choice for controlled differentiation in muscle engineering.

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Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells

Abstract

ASJC Scopus Subject Areas

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